786 
Journal of Agricultural Research 
Vol. XXI, No. 10 
previously cited, in that the microorganisms are found responsible for 
the production of acid. 
Plant enzyms evidently play an important part in the hydrolysis of 
protein, as is shown by the formation of ammonia after the silage had 
been treated with 2 per cent chloroform, which inhibits the growth of 
bacteria. The formation of amino nitrogen in the chloroformed silage 
resembles that of normal silage. Practically no formation of amino 
nitrogen was observed in silage sterilized and inoculated. It is con¬ 
cluded that the hydrolysis of protein with formation of amino nitrogen 
