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‘ I. The nature of the various varieties of “ fly ” disease parasites 
met with in domestic or wild animals in the territory; their effects 
upon domestic stock ; and the action of certain selected drugs upon 
them, more particularly the employment of atoxyl and mercury and 
the use of the more easily obtained aniline dyes. 
‘ 2. The incidence of trypanosome infection in wild game ; the 
nature and effect upon stock of the parasites found. 
‘ 3. An enquiry into the transmission of fly disease by means of 
biting flies, more particularly Tsetse, “Hippo” and "Blind” flies, 
and the “ Stinging ” house fly.* 
‘ In the event of success attending the experiments at least one 
year would be required for thoroughly testing and proving the method 
of treatment; and since these would follow on those already 
conducted near Broken Hill, we commence from a working base 
which eliminates a considerable number of tentative experiments. 
' The other main points would receive attention concurrently. 
‘ It is not to be expected that this period of time is sufficient to 
do more than elucidate a few of the many questions associated with 
biting flies and big game. One could only work from hand to 
mouth, and from deductions drawn, institute control experiments 
which might answer the questions—can flies other than tsetse 
originate an outbreak of disease? and can flies other than tsetse 
continue the transmission in a herd when once originated ? 
‘ It is known that game harbour the parasite: we could only 
ascertain figures showing the proportion of each variety affected and 
the effects of these germs on domestic stock. To ascertain these 
with accuracy a very large number of head would require to be 
examined ; but at any period of the investigation figures having 
certain values would be available. 
‘ I would then suggest one clear year as a minimum and estimate 
accordingly, leaving it to be understood that in research work it is 
impossible to precisely specify periods of time. 
The requirements of such an investigation would be: — 
A main camp situated clear of all danger from contamination 
by wandering tsetse flies, in the vicinity of a plentiful water supply 
and grazing for experimental animals, preferably within easy reach of 
Hippo = 7 'abanus | Blind = fiaematapota ; Stinging housefly = Stnmoxys. 
