19 
‘ The drug was used only upon animals showing the effects of the 
parasites, such as loss of weight, anaemia, fever, and auto-agglutination 
of the corpuscles, and no animal was used until its blood contained 
numerous parasites. The numbers of the parasites present differed 
according to the species of animal and the disease. In the majority 
of the experiments, control animals which were not treated, and had 
been inoculated at the same time as the treated animals, were used 
In all cases the control animal died. 
‘ Intravenous inoculation was used only on rabbits, all other animals 
were injected subcutaneously. Treatment was continued for one to 
three months or until increase of weight, diminution of the anaemia, 
and entire absence of parasites from the blood, as far as microscopical 
examination could determine, was noted. At various periods 
susceptible animals were inoculated with the blood from a treated 
animal. When treatment had been discontinued for one to three 
months, or longer, the animal was bled or killed and all the blood 
available was used to inject susceptible animals. Inoculated animals 
whose blood has given negative results after three to six months, or 
after longer periods, have been inoculated with virulent blood, and 
have taken the disease, thereby showing that no immunity was 
conferred by the previous inoculation. 
‘ T. gambunse.—'R2hhit, male, weight, 2,010 grammes. Parasites 
appeared on the twelfth day. On the forty-sixth day numerous 
trypanosomes were present; it had lost weight (1,890 grammes). A 
blood count gave reds, 4,980,000 ; whites, 8,860 ; haemoglobin, sixty- 
seven per cent. For three-and-a-quarter months it received ro c.c, of 
five per cent, solution atoxyl three times a week, gradually increasing 
the amount to roc.c. of ten per cent, solution. It then weighed 
2,000 grammes. The blood count was reds, 6,640,000 ; whites, 6,200 , 
haemoglobin, eighty-eight per cent. The blood in quantities of 10 c.c. 
was non-effective. The auto-agglutination of the corpuscles was lost. 
Thirty-two days later it was very ill ; it was therefore bled to death, 
and the whole of its blood injected into a monkey. This monkey 
has never become infected. The post-mortem showed severe 
haemorrhagic cystitis, the bladder in parts being almost gangrenous 
and acute septic peritonitis, especially around the bladder. The 
spleen showed no congestion, but the connective tissue was slightly 
increased. The kidneys and liver were normal. 
