9 
obtained better results with Trypan-red than with either Arsenic or 
human serum, upon mice infected with Mai de caderas. They were 
able to cure mice, and in rats the parasites disappeared, but reappeared 
after a short time. The result of these eminent pbserver^ drawing 
attention to the use of Anilin colours was to stimulate a great amount 
of investigation in this direction. 
Laveran and MesnIL, \Le irypanroth dans U traitement de 
quelques Trypanosomiases' (Comptes rendus de lacad. des sciences, 
Vol. 139, p. 19), confirm Ehrlich and Shiga. 
Halberstadter (Centralbl. f. Bakt., 1905, Vol. 38, p. 525) 
confirms Ehrlich as regards the action of Trypan-red in Caderas. 
similarly in Dourine, but had very little success in Ngana. 
Nissle (Arch. f. Hygiene, 1905, Vol. 53. p. 181) found Trypan-red 
better against Ngana than against Caderas. 
Ewald pRANitE, ' Therapeutische Vgrsuche bei Trypanosomen 
erkrankung’ (Inaugural dissertation. 1905* ^^e result of an 
extensive study of Trypan-red on T. equiperdum and Mbori strains, 
recommends especially the combined treatment of Trypan-red and 
Arsenic. 
WendELSTADT (Deutsche med. Wochenschrift, 1904, No. 47) 
found that Trypan-red administered in small doses internally caused 
the trypanosomes to disappear, but that they soon reappeared again. 
He wa3 able to cure rats infected with Ngana. 
WENDELSTADT (Deutsche med. Wochenschrift, 1907) by the 
internal application of trypan-red only succeeded in obtaining 
prolongation of life in the case of ngana-infected animals. 
THOitAS and Breinl in a paper entitled ‘Trypanosomes, 
Trypanosomiasis and Sleeping Sickness’ (Memoir XVI, 1905- of the 
Liverpool School of Tropical Medicine), published a full report of 
treatment experiments with various drugs, amongst them Trypan-red, 
but they only succeeded in demonstrating that it helped to prolong 
the life of infected animals. 
WENDELSTADT and Fellmer, in a paper entitled ‘ Uber die 
Einwirkung von Brilliantgriin auf Naganatrypanosomen ’ (Zeitschrift 
fur Hygiene und Infekt. Krankheiten, Vol. LII, 1906, pp. 263-280), 
state that the parasites disappear from the peripheral circulation, 
and that after repeated doses the blood of the animals becomes 
negative as tested by subinoculations. 
