r 
I«5 
first day the cattle accommodated themselves to this method, which 
cannot be held as interfering with the proper thermometric 
registration. Under these conditions the normal temperature of 
healthy cattle was found to be approximately 100° to 102° F., two 
degrees representing the normal diurnal variation. 
During the course of the disease the temperature was almost 
constantly elevated; to a slight degree it was paroxysmal, but, as the 
accompanying charts show, this was not a marked feature. 
Parasites could usually be found on direct examination of 
peripheral blood. For graphic representation a similar system to 
that adopted by Lingard is used, but the number of trypanosomes 
present is so much less (12 to a field (Zeiss Oc IV, Obj. D) was the 
greatest number seen) that lower values are accorded to each mark. 
When numerous it was customary to count forty fields and take the 
average; but when scanty sixty to a hundred, according to 
indications. No animal is marked ‘ absent ’ unless this latter number 
was counted; and in necessary cases, as in the treatment 
experiments, a |-inch square cover-glass was searched before placing 
a minus sign. 
There is no close relationship between the temperature and the 
number of parasites seen; sometimes a temperature of 104° or 
105° F. was unaccompanied by trypanosomes, or only one to twenty 
fields; and again, a temperature of ioi° has been seen with four 
organisms to a field. Two cases were observed in which organisms 
were not seen for two or three weeks, and in one (No. XIII) which 
was diagnosed on July i8th, trypanosomes were only seen on 
August loth (one to a cover-glass during the ten weeks the animal 
remained under observation). These might be considered as chronic 
or latent infections, though both died, apparently within the usual 
time of the disease. 
Gland puncture of the prescapular lymphatics was tried as an 
additional aid to diagnosis, though its general employment is not so 
generally necessary owing to the fairly constant presence of trypano¬ 
somes in the peripheral blood. The method is essentially that 
described by Dutton and Todd for the diagnosis of human trypano¬ 
somiasis, all specimens being sealed with vaseline and examined 
immediately. It will be seen from the figures below that it is of less 
value than blood examination as a means of diagnosis, but its 
