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of the spleen is inconstant, but it is usually friable with prominent 
malpighian bodies, and the capsule studded with petechiae. In only 
one case, No. XLI, showing marked nervous symptoms, were any 
gross changes seen in the spinal cord. 
Trypanosomes were not constantly seen in the blood at death, nor 
were films, made direct from the lymphatic glands, invariably positive. 
Those, however, made from the haemorrhagic lymphatics of the 
mesentery possessed greater diagnostic value than the others, and 
owing to the danger of a negative diagnosis being made on a blood 
film sent in for opinion, we would recommend the forwarding of one 
made from a haemorrhagic gland in addition, though it must be 
understood that it is necessary for this to be made as quickly as 
possible after death in order to avoid undue phagocytosis. 
These cattle, compared with those of India, are singularly free 
from organic changes in any organ, and from other animal parasites. 
In most (i6 in 22) there were a few Filaria papillosa, and in many 
(10 in 22) Paramphistomum conicinn was seen. Distomum 
hepaticum was not encountered despite the prevalent idea that 
‘ fluke ’ occurs here. No filarial embryos were seen in the blood, and 
Piroplasma bovis was only seen on one occasion in two animals. 
(2) The experimental disease. — The strain of T. dimorphon 
mainly used in these experiments was derived from a naturally 
infected cow, Case No. XXV. This animal was selected as 
representative of the disease; as .she had been confined to the 
homestead and had not travelled on the surrounding roads, the 
danger of a mixed infection being thereby reduced. Though 
apparently in perfect health, she had, owing to her contact with the 
sick, been subjected to four examinations between June 26th and 
July 26th, when she was brought in for daily observation. Trypano¬ 
somes were first seen on July 29th, and it is believed that she 
contracted the disease on the farm. The same strain passed through 
Case No. XLII served for most of the other inoculations. 
I. Cattle. Both the animals inoculated with virulent blood 
were later utilised in the experimental work on treatment, which was 
regarded as of more direct importance. Organisms appeared on the 
seventh and eighth day following inoculation, and remained constant 
until the exhibition of atoxyl, in one case, No. XLII, for 24 days. 
Death in the case of No. XLI was largely due to atoxyl-intoxication. 
