Ill 
remained normal until the morning of the 21st when it commenced to rise, 
continuing to do so gradually till the morning of the 26th when it was 105° F. 
That evening it was 102®, and it kept normal for three days to “rise again for the 
second paroxysm on the 30th. Trypanosomes appeared on the third day after the 
temperature rose, August 23rd, one to a cover-glass, and increased progressively 
to the 25th, one to six fields. On the 26th, 27th, 28th, they were not seen ; they 
reappeared on the 29th, and were twelve to a field on September ist. They 
gradually fell in number towards death, which took place on the night of the 4th. 
(vide chart vi.) 
Case No. XLIII. — A ‘kaffir' pup, aged three months. August 20th, 
inoculated 0-5 c.c. citrated blood of XXV. The temperature was very irregular 
throughout, varying from 96*4'’ to loj-S®. Trypanosomes appeared on the seventh 
day, and remained constantly present till death on the fifteenth day. During the 
last three days they were very numerous, ten to a field, and the temperature was 
usually subnormal. 
Case No. LIV.—Ilrother to the last. September 8th, inoculated 
subcutaneously with 1*0 c.c. citrated blood of XLII. The temperature showed 
considerable daily variation. Organisms appeared on the eleventh day, September 
t4tb, one to five fields, and remained present in slightly increasing numbers, till 
death on the eighteenth day after inoculation, September 21st. 
Post-mortem affearances . — In all three animals the most noticeable feature was 
a considerable enlargement of the spleen, which in the adult dog (weight about 
30 lbs.) measured 33 cm. in length, to cm. in width, and 4’5 cm, in thickness ; dark 
in colour, sofe and friable and edges rounded. Lymphatic glands of the mesentery 
enlarged congested and haemorrhagic. Petechiae studded the serous membranes; 
the liver pale and friable. The pericardial sac contained from 20 to 75 c.c., and 
the peritoneal cavity of Case LIV 250 c.c. ; the amount in the thoracic cavity was 
not greatly increased. 
6. Rabbit. Only one animal was inoculated. The disease in 
this was of chronic nature, and organisms were rarely seen in an 
ordinary cover-glass examination. 
Case No, XXXVII. — August 7th, inoculated intraperitoneally with i*o c.c. 
citrated blood of XXV. During the four weeks during which it was taken, the 
temperature showed no gross variations. Organisms appeared on the tenth day, 
one to a cover-glass, and on the following day five to a cover-glass were seen. 
They were again detected on the fourteenth, fifteenth and thirty-second day after 
inoculation 5 from this time they were not seen on the daily examination up to 
September 30th, nor on those made about every fifth day since. During the first 
month of disease the rabbit lost condition ; hair began to come out at the base of the 
ears, around the eyes, and on the rump, and sores formed on both tarsi. No 
oedema was noted, nor did any opacity of the cornea or signs of paralysis occur, 
During the next three months the rabbit appeared to improve in condition, and the 
tarsal ulcers dried up. Towards the last fortnight a slightly purulent discharge 
collected on the nostrils and around the eyes. Death took place in a very emaciated 
state on January 21st, 1908, 168 days after inoculation. 
Post-mortem .— The spleen was considerably enlarged and rounded, but firm in 
consistency, the mesenteric lymphatics swollen but pale. Trypanosomes could not 
be found on direct examination ; no sub-inoculations were made. 
7. Guinea-Pigs. Five guinea-pigs were inoculated with this 
trypanosome. In all, the disease was of a rapidly fatal character, the 
