228 
precautions were taken. The mouth-part of the pipette contained 
KOH between two layers of cotton wool, so that all the CO, of the 
breath was absorbed by the potassium hydroxide. 
The blood was collected in small test tubes and left standing over 
night in an ice chest; then the serum was separated from the clot and 
used for the estimation of acidity. For the estimation of the 
alkalinity, the blood was collected in a platinum crucible and 
incinerated. All the glass vessels used in this work were immersed in 
strong hydrochloric acid for three days, and then in distilled water for 
the same length of time, so as to avoid the error which might be 
produced by the alkalinity of the glass. 
Experiment No. 303. Rabbit, male, inoculated on loth February, 1908, with 
February the acidity wa.s 0*03015* (phenolphthaleinei ; m 
(rnncT ^ had increased to 0*03255 {phenolphthaleinei, 0031Q5 
(Congo red), the alkalinity was 0*02895. The animal was frequently examined aod 
/nhpnni* vTfk ^7 until 7th April, when the acidity had reached o-o3495 
(phenolphtbaleine), 0*03345 (Congo red), and the alk.-,]inity 0-03015. 
T ^ female, inoculated on loth Februaiy’, 1908, with 
j February the acidity was 0*03285 (phenolphthaleirei, 
reaihii alkalinity 0*02865, On 7th April the acidity had 
It i<! r caolphthaleinej, 0*03405 (congo red), and the alkalinity 0-02925. 
phenol, . I,tWeire^vera^ha Iama,°o.a346s.““'"'’ 
Ouinea-pig, female, inoculated on 24th February, 19°^- 
Fehninrv ,1. ^ ®*^^^*city and acidity estimations were made on 25 th 
(co.igo red) and O'OJioS (phenolphtbaleine), 0-03075 
be swarming o'o 3045 - On nth March the animal was found to 
o ''’3435 (phenolnhth acidity estimation on 14th March gave 
alkalimV 0-03000. The 
made twentv rr.- ♦ ^^^ch of typical trypanosomiasis. The acidity estimation 
(Congo TeS;!"^ 0-036,5 (phenolphtbaleine), and 0-03# 
with T. brucei. female, inoculated on 24th February, 190*. 
be o-o3iqc (phenolnhthaf^-^ estimation wa.s made on 26th February, and found to 
animal sLwed """ alka.ini? 0 03030. 
reached 0*03465 (phenolDhtha1e;°"i 
the animal was then heavily infec4d'°^^^° (congo red), and the alkalinity 0-02925: 
Experiment No 210 _ r, • 
with T. brucei. On 26th p female, inoculated on 24th February, i 9 »' 
0-03210 (congo red), and the^Ju^v^ acidity M*as 0*03225 (phenolphthaleinei. 
°-03405 (phenolphthaleinei cL ^ 1 °'°'*S95. On 8th March the acidity war 
14th March. The acidity reach^H^ (congo red). A fall in the acidity was noticed on 
Congo red. Afterwards the arlrfi* (phenolphthaleine), and the same also for 
23 fd 0*03510 (phenolphthaleinei ^ ® f® increase and became on March 
On 7th April the acidity had ’ °‘°3375 (congo red), and the alkalinity o-orgSi- 
red), and the alkalinity 0-03045.*'*^''^®'^ (phenolphtbaleine), o-o 3495 
* Expressed 
