229 
Experiment No. 3ii. -Guinea-pig, male, inoculated on 24th February, 
wilhf brucci. On 24th February the acidity was found to be 0-03245 (phenolph- 
Talie. 7 o 3;25 (congo red), and the alkalinity 0-03000. The last estimation was 
made on’ 17th March, and the animal died on 19th March. The ^ 
slishUy infected, and the acidity found to be 0-03300 (phenolphthaleinel, 0 03255 
;io«rand the alkalinity 0-03000. The cause of the death of this animal was 
pneumonia. , , 
Experiment No. 314.—Rabbit, female, inoculated on t4th Match, 1908, with 
r brucei. On the day of inoculation the acidity was found to °^3075 
iphenolphthaleine), 0-03030 (congo red), and the alkalinity 0-02850. On 23rd Marcn 
the acidity was found to be 0-03300 (phenolphthaleine), 0-03195 (congo red), 
alkalinity 0-03060. On the same day, three young ones were born which Mon died. 
The aciditv on 2Ctb March had dropped down to 0-03240 (phenolphthaleine), 
0-03225 (Congo red), and the alkalinity 0-02850. From this day the acidity 
increased slowly, and on 7th April was 0-03345 (phenolphthaleine), 0-03285 (congo 
redi, and the alkalinity 0-03015. The animal died on 9th February. This anima 
was positive all the time, starting from 17th March. 
Experiment No. 351.—Rabbit, female, inoculated on 14th March, 1908, with 
T. brucei. The acidity was then 0-03120 (phenolphthaleine), 0-03075 (congo red), and 
the alkalinity 0-02940. On April 7th the acidity had reached 0-03375 (phenolpti- 
thaleine), 0-03315 (congo red), and the alkalinity 0-029:0. 
Experiment No. 316.—Rabbit, male, inoculated on 14th March, 1908, wit 
r, brucei, showing an acidity of 0-03180 (phenolphthaleine), and o 0^75 » 
and an alkalinity of 0-02880. When examined on 31st March, the acidity ^ 
reached 0-03375 (phenolphthaleine) 0-03210 (congo red), and the a a ini y o 03 
Experiment No. 3i7.-Guinea-pig, female, inoculated on 14th March, 1908, 
with T. brucei. The acidity was then 0-03150 (phenolphthaleine), 0-03105 
red), and the alkalinity 0-02895. The animal died on 8t ° . uinpl 
trypanosomiasis On 7th April the acidity had reached 0-03585 (phenolphthaleine), 
and 0-03360 (congo red), when the alkalinity was 0-02850. 
I should like especially to draw attention to this experiment, m 
which both the increase of the total acidity and also that of the 
amido acids is very marked. 
The following table gives in full the changes in acidity and 
alkalinity during an experimental infection : 
Experiment No. 304.-Rabbit, male, inoculated loth February, 1908, with 
Alkalinity to 
dimethyl 
r . 
Acidity to 
Acidity to 
Date 
phenolphthaleine 
Congo red 
12/2/08 
0-03*35 
14/2/08 
0-03195 
... 
18/2/08 
0-03225 
. .. 
20/2/08 
0-03285 
0-03195 
24/2/08 
0-03240 
0-03210 
27/2/08 
0-03240 
0-03225 
2 l 3 loi 
003255 
0*03210 
4/3/08 
0-03300 
0-03210 
8/3/08 
0-03255 
0-03225 
10/3/08 
0-03285 
... 0-03210 
*4/3/08 
0-03255 
0-03210 
*7/3/08 
0-03255 
0-03255 
*9/3/08 
0-03285 
0-03225 
23/3/08 
0-033*5 
0-03225 
.—Trypanosomes examinations 
were made daily. 
0-02895 
0-02880 
0-02880 
0-02895 
0-02865 
0-02895 
0-02865 
o- 02880 
0-02910 
0-02985 
The animal died on 
