234 
appearance of the parasites, the peripheral and especially the blood of 
the organs was usually teeming with them, and the animal succumbed 
to the infection. The course of the disease in full-grown dogs was 
somewhat modified, as the parasites only appeared after a prolonged 
incubation period, and never in such large numbers as in young 
animals. 
The clinical feature of this disease has been dealt with in full by 
previous workers. It is a noteworthy fact that in nearly all our cases 
haemoglobinuria was more or less pronounced, the urine being 
frequently of a dark port wine colour. Only in very few cases was 
jaundice well marked. 
Technique. All our observations were made on wet films using 
BreinI s metliods. The blood-smears were fixed in strong Flemming's 
solution, and afterwards stained with safranine and methylene blue, 
according to BreinI s method, or his modification of HeidenhaiD,‘= 
using as counter-stain a dilute solution of Bordeaux red. By this 
means the cytological details of the parasites were well preserved, an 
attainment which is impossible by any dry film method. 
EARLY FORMS OF PARASITES IN THE 
he early forms in the blood are usually very large and irregular, 
requently exhibiting pseudopodia of varying form and size (figs. 1-5)- 
e o these processes are so fine that they simulate flagella, and at 
imes small particles of protoplasm appear to become detached; but 
in most instances a very fine band may still be detected connecting 
itt e masses with the parasite. The protoplasm consists of a 
air y coarse spongioplasm (‘ Schaumplasma ’), containing fine, bluish 
s ainmg granules, embedded in its substance. At this stage the 
P s tes usually possess a single nucleus, in the form of a small, 
j staining mass of chromatin, which is sometimes 
div' ■ ” ^ filled with lightly staining substance. The 
1 T" I " nucleus of the 
mr. f ^ afterwards separates into two halves which 
ve ur er apart ; meanwhile the parasite itself increases in size, 
cells C%s. .-4). This 
process goes on very rapidly. ^ ® ' 
In the early forms very rarely a second smaller chromat.c mass is 
