^35 
present (fig. 5), which may be connected with the mam nucleus by a 
line darkly staining line. If Breinl’s stain is used this smaller nucleus 
usually takes a dark purplish-blue colour, whereas the nucleus stains 
dark red. This difference is noticeable, however, only in well stained 
specimens. 
The division of the later forms proceeds in a different way. The 
small nucleus together with the chromatic line usually divides first. 
A median cleft afterwards appears, which extends between the two 
small nuclei In the meanwhile the large nucleus elongates, and 
finally separates into two equal halves. The two daughter cells then 
become separate (figs. 6-9). This division results in the formation of 
Uo pear-shaped parasites. 
At this stage of the infection, round parasites, now and again 
reproduce by budding, a process which becomes more frequent as the 
infection advances. The nucleus throws out a portion of its 
chromatin, wliich moves outwards, but remains connected with it by 
means of a thick band. As this chromatic mass approaches the 
periphery of the parasite, the cytoplasm bulges out from the surface 
and concentrates itself around the terminal enlargement of the 
chromatic band. The latter structure becomes thinner and finally 
breaks. The connection of the bud with the main mass becomes in 
the meanwhile less and less extensive, and finally the bud is detached 
(figs. 10-12). Very often two buds are formed at the same time in a 
similar manner (figs. 13-16). A large number of buds as described by 
Kinoshita^ have never been distinctly observed by us. 
Schaudinn^i and Luhe^^ were the first to draw attention to the 
presence of a small nucleus in Piroflasma. This discovery has been 
confinned by different workers. Schaudinn named the second nucleus 
^ ^^lepharoplast, and most of the later workers adhere to this view, 
"Without, however, producing any evidence in support of it. 
Our observations show that but few binucleate parasites are 
present at an early stage of the infection. This small nucleus then 
seises from the large one, usually at a later stage of the disease, 
different phases of this process may sometimes be seen in one red 
Wqtuscle containing several parasites (figs. 17, 18, 42). The nucleus, 
'^hich at this stage is surrounded by a vacuole, buds off a small part 
its substance, which moves to the edge of the vacuole, often leaving 
^ thin connecting line behind 
