236 
LATER FORMS OF PARASITES IN THE BLOOD 
As the infection advances, the parasites undergo marked changes, 
and only now and again large amoeboid forms are seen, The 
parasites diminish in size, and are frequently pear-sliaped. The proto¬ 
plasm, which at first is a typical ‘ Schaumplasma,' becomes much 
denser in structure. The percentage of binucleate forms increasej, 
and many free forms are encountered. 
A peculiar feature of this stage is the detachment of small parts 
of the cytoplasm in a definite way. At one side of the cell appears 
a vacuole, which increases in size and enlarges within the parasite, 
until the protoplasm is almost separated into two unequal parts, which 
finally become separate. The smaller part is entirely cytoplaanic 
in nature (figs. 22-26). 
Owing to the rapidity with which multiplication takes place, the 
nuclear details become very irregular, and frequently a second division 
commences before the completion of the first (figs. 20, 51-52). 
The nucleus of the round forms is usually surrounded by a 
vacuole (fig. 33). The division is by simple fission, in which the 
nucleus divides with the vacuole (figs. 33-38). 
Sometimes the parasites assume a signet-ring form, a large vacuole 
occupying the middle of tlie cell, the nucleus which lies at the 
periphery often dividing (fig. 21). 
The usual mode of division at this stage results in the formation 
of two pear-shaped forms, but differs from that in the early stages of 
t e disease. Starting again from the round binucleate form, either 
t e arge or the small nucleus divides, together with the line; the 
sina nucleus moves to the edge of the parasite, and the chromatic 
ecomes fainter, and in many instances finally disappears (figs, 
r/ ^ divided large nuclei frequently remain connected 
^ 7 \h n ''^‘^uoles appear about the middle 
^1. Tu’ ^^’crease in size. Often the two parasites are 
nec e y t ree fine protoplasmic strands, two peripheral and one 
cross the middle, a large and small nucleus in each half. First the 
centra connecting strand breaks, and the separation of the two 
parasites becomes more pronounced, until they are only connected by 
coL Tf- ""V u ‘he pear-shaped forms are stiU 
connected in the above described manner, the connecting cytoplasmic 
