IP 
269 
III. INEFFICACIOUS SUBSTANCES 
Because of the marked action of quinine on other protozoa, it 
was thought that substances derived from it, or resembling it, might 
be trypanocidal. Since Atoxyl. an organic arsenic-containmg 
compound, kills trypanosomes, other compounds of like nature were 
tried alone, or in combination with various drugs, various ot er 
substances were tried in the same way. None of them were of value 
The following are the drugs and combinations of drugs tried withou 
advantage on rats infected with Trypanosoma brucei-. 
Quinine. 
Cinchonine. 
Cupreine. 
Quinine-cacodylate. 
Quinine-cacodylate followed by Sublimate. 
Quinine-cacodylate followed by Iron-cacodylate. 
Quinine-cacodylate followed by Iron chloride. 
Arrhenal. 
Potassium bichromate. 
IV. ANILINE COLOURING MATTERS 
Our work m th.s direct,orr .s st.U very incomplete ■ ^ 
however, what results we have obtained. We have adopted the 
following routine method of gaining an idea of the efficiency of an 
uutned substance. A series of rats is inoculated; some a e l ft 
untreated, others are given one full dose of substance to be^trmd 
The average difference in days between te ^a e indicated 
controls and of the treated animals is no ■ /mlnmn i)' 
for each of the drugs mentioned m the following '-t (column 
our usual virulent strain of Tryfanoscna bruce, was the infecting 
parasite in each case. 
Average of 6 rats 
Average of 9 rats 
Average of 4 rats 
Phenolphthalein 
Acetylated Phenolphthalein 
Acetylated Phenolphthalein 
in alkaline solution 
Methylated Phenolphthalein 
Fluorescein 
Acetylated Fluorescein 
o days 
2-3 days 
3 days 
Average of 2 rats 
Average of 4 rats 
Average of 3 rats 
o days 
o days 
1 day 
