274 
5 c.cm of a len per cent, solution of acetylated Atoxyl. The parasites disappeared 
from the blood on the fifth day of the experiment and were absent until the deati 
of the animal on the forty-seventh day. 
A rat was inoculated on the seventh and another on the twenty.founh day of 
the experiment; neither of them ever became infected. 
ExPEKi ME.vT 420 .—Trypanosomes appeared in the peripheral blood on the third 
day after inoculation. On the fourth and again on the fifth day 2 c.cm. of a 
five per cent, solution of acetylated Atoxyl was given. The parasites disappeared, 
and although they were still absent on the ninth and three succeediog days, 
13 c.cm. of the same solution was given in four doses of increasing size; no toxic 
symptoms were observed ; and on the fourteenth, and again on the fifteenth day 
2 c.cm. of a 0*5 per cent, solution of .Mercury bi-chloride was given. The 
parasites still remained absent, when on the twenty-ninth and thirtieth days two 
more doses of 5 c.cm. of the same solution of acetylated Atoxyl were injected and 
were foHowed on the two next days by two doses of 3 c.cm. of the same solution 
of Sublimate. The parasites remained absent until the death of the animal on the 
one hundred and fifth day ; death was due in a large measure to verv extensive 
mange. 
On the twentieth, forty-first, and sixty-seventh days of the experiment 
respectively, two rats and one puppy were inoculated with large quantities of 
blood ; none of them ever became infected. 
Experiment 432. — Trypanosomes appeared in the blood on the second day- 
On the third and again on the fourth day 3 c.cm. of a five per cent, solution of 
acetylated Atoxyl was given; a dose of 5 c.cm. of a ten per cent, solution of 
acetylated Atoxyl was injected on the tenth and again on the eleventh day. This 
dose was repeated on the seventeenth and eighteenth days and was then followed 
on the nineteenth and twentieth days by two doses, each of 2 c.cm. of a o-j pet 
cent, solution of Mercury bichloride. The parasites disappeared from the blood 
on the fourth day of the experiment and never reappeared. The animal died on 
the thirty-seventh day. 
A rat was subinoculated from this animal on the sixteenth day ; a rat and two 
mice on the twenty-eighth day, and a rat on the thirty-fifth day; none of these 
animals ever became infected. 
Three dogs treated by acetylated Atoxyl lived from thirty-seven 
to one hundred and five days. 
1 ^- Guinea Pigs. 
*. .t rtuiuieni oy nioxyt atone. 
On — Trypanosomes appeared in the blood on the fourth day. 
nar, ■. ' c.cm. of a five per cent, solution of Atoxyl was given and the 
parasites disappeared, but death ensued on the seventh day. 
the 4obb — Trypanosomes appeared in the blood on the sixth day. Oa 
Atoxvl wa eighth day i c.cm. of a five per cent, solution of 
day ^ given. The parasites disappeared, but the animal died on the ninth 
On each^ont^"^. ^I’ypaiosome.s appeared in the blood on the eighth day- 
was days . c.cm. of a five per cent, solution of-Atoxyl 
the fifteenth'' day disappeared from the blood, but death followed on 
^riimals treated by Atoxyl alone died in from seven to 
fifteen days. 
