279 
Atoxyl followed by bichloride of Mercury, one died in forty-six days 
and four were apparently cured ; this combined treatment of rabbits 
infected with Trypanosoma brucei therefore is much superior to the 
treatment by Atoxyl alone, and will sometimes effect a cure. 
(i) Rabbits infected with Trypanosoma 
gam b ten s e 
Expeeimext 323.—Trypanosomes appeared after forty-one days in the blood 
of a rabbit inoculated on February 26th. Treatment was commenced sixteen days 
iater and 7 c.cm. of five per cent. Atoxyl solution was given, in four doses at equa 
intervals, during the next twelve days. This was immediately followe uring 
the succeeding ten days by 4 c.cm. of bichloride solution, given in our equa 
doses at approximately equal intervals. The rabbit s blood was examine 
practically daily for one hundred and forty-one days, until October 5th, w eri i 
died (cause not known; no autopsy). Trypanosomes were never seen in it a er 
the treatment was stopped, nor in mice subinoculated from it thirty ays rom 
that time 
Experiment 326.—After eighteen days trypanosomes were found in the ear 
blood of a rabbit inoculated on February 26th, 1907. The parasites were con¬ 
stantly present when treatment was commenced forty-three days later, and a c.cm. 
of five per cent. Atoxyl solution was given in four doses at approxim^e y equa 
intervals during the next fifteen days. This was immediately followed j 4 c.crn. 
of Mercury bichloride solution, given in four doses at almost ^ 
during the succeeding ten days. The rabbit’s blood was examine 
forty-nine days and at intervals of two*or three days until January 2 , 15 , 
eleven months after inoculation, when it was Killed. Trypanosomes were never 
seen in it after treatment was stopped, nor in the three rats and two mice sub- 
inoculated from it just at the end of treatment and a month after treatment na 
stopped. 
Experiment 327.-After thirty-one days trypanosomes were found m the 
peripheral blood of an adult male rabbit inoculated on February 22nd, 1907. 
ment was commenced thirty days later, and 8 c.cm. of five per cent. oxy so 
was given in four doses during the next twelve days. This ° 
succeeding ten days by 4 c.cm. of one per cent. Mercury bichloride f 
given in four equal doses at almost equal intervals of time. e ra , 
was examined daily after the cessation of treatment for one hun re an ^ , 
four days and, after that, at intervals until February 3rd, 1908, over e 
after inoculation, when the animal was killed because of severe s m 1 
Trypanosomes were never seen in its blood after the treatment was s °PP® ’ 
ic mice and rats subinoculated, respectively, a month after t e s opp g 
treatment and at the autopsy. . 
Experiment 329.—After twenty-six. days trypanosomes appeare 
peripheral blood of a rabbit. Treatment was commenced thirty-two ^ays ^ > 
and II c.cm. of five per cent. Atoxyl solution was given m five ‘^°ses during 
next twelve days ; just over half of the drug was given in the last two bay • 
was followed during the next nine days by 7 c.cm. of bichloride ^ 
four doses ; just half of this drug was given on the first two days, 
of the treatment, the rabbit’s blood was examined daily for one hundred bay^’^hen 
h died (cause not known ; no autopsy). Trypanosomes were riever ^ 
blood, nor did they appear in the blood of a mouse inoculated at the 
of the treatment nor in rats inoculated at respectively thirteen and seventy-four 
days from that time. 
