2^0 
The disease produced in rabbits by Trypanosoma gatnbitHst ss&'j 
run a very chronic course; it is therefore better to say nothing 
concerning Experiments 323 and 329. Experiments 326 and 327 
may, however, be safely considered to have been cured of an infection 
by Trypanosoma gambiertse. 
B. Donkeys. 
The outcome of this series of e.xperiments was extremely 
disappointing. It was commenced immediately after the favourable 
results had been obtained in the treatment of rats by means of 
combined Atoxyl and Mercury bichloride. There is no doubt that in 
the first of the succeeding experiments the disease was allowed to 
go too far before the commencement of treatment; but even when, 
as in the later experiments, treatment was commenced early, it was 
found impossible to save a single animal. 
In all the donkey experiments, as a general rule, the temperature, 
taken twice daily, became higher when the trypanosomes reappeared 
in the peripheral blood ; whether trypanosomes were present or not 
it was often noticed that an elevated temperature fell immediately 
after the administration of another dose of the drug, and it w’as 
frequently observed that animals inoculated at such a time, that is, 
when the examination of the blood was negative and the temperature 
was high, often became infected. As a rule, therefore, a high 
temperature was accepted as a definite indication for a dose of Atoxyl 
Controls, treated by Atoxyl 
1 he disease was allowed to run its course, without treatment, in 
tv\o donkeys; trypanosomes appeared in the blood on the third day, 
and they died after a typical illness, with remitting and intermitting 
fever going up to 104° or 105^ F., in eighteen and twenty-four days 
respectively; at the post-mortem typical signs of trypanosomiasis 
'vere present. 
Treatment was commenced on the twentieth day in a third 
animal, inoculated at the same time, when it was already practically 
moribund ; it died on the twenty-third day. 
9 rrypanosotnes appeared four days after inoculation- Th®>' 
sixth dav wh slowly and treatment was not commenced until the 
dose wafren ? ^ «°l“tion of Atoxyl was given. Tlus 
peatedonthethirty-second and thirty-third days because of a recurrence 
