282 
course. The teraperalure of this donkey, although irregularly febrile, did acKnifl 
as high as was usually the case; it did not go above after treatmem *15 
commenced. 
Experiment 14. — Trypanosomes appeared in the blood on the filth daj, 
Treatment was commenced on the seventeenth day by injection of 10 c.ctn- d 1 
ten per cent, solution of Atoxyl. ThTs .same dose was repeated daily—SundaTs 
excepted—from then until the ninety-fourth dav of the experiment. The tiypaao- 
somes disappeared from the blood after the 'first dose of Atoxyl and did no: 
reappear until the seventy-fourth and seventy-fifth days, when they once moti 
disappeared until the ninety-fifth day. They disappeared once more and were n« 
seen again before death on the one hundred and fourth day. Rats subinocoUted 
just before the second dose of Atoxyl never became infected, while rats inoculate: 
on the fifty-fourth and sixty-eighth days both became inftcUi after incnbatinc 
periods slightly longer than usual and died in due course. 
From these experiments it appears that dailv' doses of 10 cm 
of a ten per cent, solution of Atoxyl, and occasionally twice thal 
amount, could be g;iven to an adult donkey without exciting acute 
signs of poisoning; but even these large doses were insufficient to 
keep the trypanosomes out of the blood and the animals invariably 
died, whether the parasites were to be found in the blood or no, in 
from twenty-three to one hundred days, usually in about fifty days. 
2. Treatment by Atoxyl and bichloride of Mercury. 
{d) Symptomatic treatment 
Iri the following series of experiments treatment was given 
practically only when it seemed necessary because of the presence of 
trypanosomes, or of a high temperature. 
At first weaker solutions of Atoxyl were used; as it became 
apparent that they were inefficacious they were strengthened, until 
the saturated twenty per cent, solution was currently employed; 
this same strength of the solution was used in all the later 
experiments. 
after 
.^’‘’.ypanosomes appeared in the blood five 
iniection M ° ®*g’hfh and ninth days treatment was commenced by the daiy 
thirteenth ^ of Atoxyl ; on the twelfth 
given • on th^fA ^ sblution of Sublimate 
On the fifteenth”*^ ®fnth day four times that amount of the drug was administer 
.0 com. 0, 'wer.,y.„i„,h, thirty.fourlh aad thirty-fiflh 
on the second 'Ejected. The trypanosomes disappeared from the b 
in tempeXe buTth were one or two considerable elevahoes 
ninth day when ro P®'‘®sites remained absent from that time until the twrt 
bichloride TuZury rt cl 
this treatment hnf ^ parasites disappeared from the blood as a resu 
®atment. but reappeared on the forty-sixth day. Three daily doses of .0 
