284 
appeared ia ihe blood oa tie acoad 
Ireatment was comaienced on the seventeenth dav. The parasites ehict 
recurred earl>- jn the treatment, were absent for six weeks toward its end, but tier 
eventually reappeared and the animal died on the one hundred and twentj-secoDd 
A rat subinoculated on the twenty-eighth day became infuUi after jn 
uioculation period of 23 days, although trypanosomes had not been seen in ibe 
blood for a week and the treatment of three doses of .At'oxyl and two of Mernir 
bichloride had only ended five days previously. The temperature was not 
199*2 1, and trypanosomes did not appear in the blood for two weeks later. 
KxPERfjfENT 24.—1‘arasites appeared in the blood on the third day. Trtii- 
men was commenced four days later. The parasites were very persistent at Ibe 
•rif experiment, although they were absent during the last three weeks 
ihe animal died on the thirty-third day. 
Two rats subinocuJated while the parasites were absent, on the dav of death 
and ten days previously, both became infecUd. 
Kxperimknt 26. Parasites appeared on the twenty-second dav. Treatment 
commenced three days later. Death followed on the forty-second day. 
(J>) Routine treatment 
Since symptomatic Irealiiient laiied, the continuous, routine 
administration of large doses of both drugs was adopted as a last 
resort; it, too, failed. 
i K ‘9 and 21. — The treatment after the coramenceuieiit was the saute 
n o ese animals. A dose of 10 c.cm. of a twenty per cent, solution of Atoxyl 
as given on the first and second days, and was followed by 10 c.cm. of a two pet 
cent, solution of bichloride of Mercury on the third day. This treatment was 
repeated twice weekly; on Sundays no treatment was given. 
trypanosomes appeared in the blood on the fourth day. 
f commenced on the twenty-fourth by three successive doses of 
thpri,-^" ^ solution of Atoxyl. Treatment as described above was 
whirh A until the one hundred and sixth dav, when the parasites, 
Trvnanr^«», absent since the second day of treatment, reappearei 
on CIV doses of 10 c.cm. of a one per cent, solution was then given 
on the parasites failed to disappear and the animal died 
on the one hundred and twentieth day. 
inoculal^H ! day did not become infected, while oat 
Darasifec K ^ nmety-third day, thirteen days before the reappearance of the 
parasrtes. became ,nfected after an incubation of seven days. 
inent trypanosomes appeared in the blood on the third day. Treat- 
seven doses of °of ‘’rt' 
parasites whiVt, ° •®"^* ® Per cent, solution of Atoxyl were given. 
during this coursf doses of Atoxyl, reappeared twice 
per cent, solution of Atoxyl was followed by i6c.cra. of a 
From the forty-sixth ®‘'®" doses, on two consecntive da) _ 
above was civen r- 1 ^ ^^® °"® hundred and fifth day the treatment indicated 
to examinaUon anf ^^® Parasites disappeared at once and remained absent 
reappeared ‘he ninetieth dav, when they once more 
twentieth day. constantly present until death on the one hundred and 
first course of treitmoro°*®- l!*®* ^ subinoculated with blood taken during tb* 
became infected affAr -ktoxyl, although trypanosomes were present, ony 
alter an incubation of .sixteen days. 
