285 
Esfekiwest 2o.-Trypanosoiiies appeared on the fourth duy. On 
uealioent was commenced and was continued until two days before death o. the 
•hirtyeighth day. For two days lo c.cm. of a twenty per cent, solution of Atoxy 
wasi daily and on the third day to c.cm. of a two per cent, solution of 
bichlLc of Mercury was administered. This treatment was repeated twice 
w«kly. The trypanosomes disappeared from the blood five days after treatment 
was commenced and never reappeared. h„,.a„,p 
\ rat subinoculated on the thirty-fourth day. four days before death, became 
infecld after a long incubation of twenly-three days. Another rat subinoculated 
oa the seventeenth day never became infected. 
Donkeys treated by Atoxyl followed by bichloride of Mercury all 
died in from about thirty-three to two hundred days (average one 
hundred days), in spite of large doses. 
Vni. TREATMENT BY TRYPANROTH FOLLOWED BY 
BICHLORIDE OF MERCURY 
This series of experiments was undertaken to ascertain whether 
Sublimate would have the same value when given after another 
trypanocidal substance as it possesses when following Atoxyl. There 
was a slight difficulty in carrying out these experiments, since 
Trypanroth was not always able to prevent an early death from the 
disease. 
Experiment 8oo.— Trypanosomes appeared in the blood of six rats 
day after inoculation. A dose of i c.cm. of a one per cent, so u ion yp 
was at once given and was repeated daily on the ten o ^ . 
parasites had disappeared from each of the rats on the sixth day o e P 
Three of the rats then received on the three following days doses ° 
a one per cent, solution of bichloride of Mercury ; three recen e no , . 
inent. The parasites reappeared in all six rats and deat o owe . 
to four days Those which received only Trypanroth died m six een, ^eventee , 
and eighteen days; those which received Trypanroth and bublinia e in 
nineteen, and twenty-two days. 
Experiment 802.— Trypanosomes appeared in six rats on the third day 
nioculation. One c.cm. of a one per cent, solution of 1 rypanroth was a ° » 
and was repeated on the six following days. The parasites disappeare ^ 
Wood on the eighth day of the experiment in five of the rats ; one le • 
tees, on consecutive days, of .-5 o-cm. of a one per cent. ““X, 
bichloride were then given to three of the rats; the remaining wo a / 
treatment. The parasites ultimately reappeared in all of and death followed 
as usual in from two to four days. Those which received only Trypanroth d ed 
in fifteen and sixteen days from the commencement of the experiment; those wnicn 
received the combined treatment in fourteen, twenty-five, and forty-eig ay.. 
From these experiments it .seems that combined treatment by 
Trypanroth, followed by bichloride of Mercury, is superior to treat¬ 
ment by Trypanroth alone, hut it is .far inferior to the treatment by 
Atoxyl and Mercury. 
