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IX. SUMMARY AND CONCLUSIONS 
I. Certain definite rules which must be followed in the 
experimental therapeutics of trypanosomiasis are insisted upon. 
II . The after history of the rats dealt with in our first paper: 
(^’ “*) is referred to. The Atoxyl-treated controls all eventually died 
of trypanosomiasis; those treated by Atoxyl followed by bichloride 
of Mercury, and by Ato.xyl followed by Donovan’s solution, which 
were tiiought to be cured, have never had recurrences, but they were 
not immune to re-inoculation. 
Ill and VI. Several drugs and combinations of drugs found to be 
inefficacious in the treatment of infected rats are named. 
IV. None of the colouring matters employed were of much 
\'alue ; it is suggested that the active radicle in trypanocidal anilim 
derwatives is the ‘ trypanophobe ’ group, NH^. 
V. In the treatment of dogs, guinea pigs, and mice, the 
comparative value of (i) acetylated Atoxyl followed by bichloride 
of Mercury, (2) of acetylated Atoxyl, and (3) of Atoxyl, is as the order 
in which they are named ; none of these methods is usually able to 
definitely cure well-established infections in these animals. 
VII. A. Atoxyl followed by bichloride of Mercury is found to 
be much superior to Atoxyl alone in the treatment of rabbits infected 
with 7 rypanosoma hrncei ; the latter is also effective in the treatment 
of rabbits infected with Trypanosoma gambiense. 
If. Ato.xyl and Mercury combined are distinctly superior to 
Atoxyl alone in the treatment of donkeys infected with Tryfanosonia 
brucei, but neither method is able to cure a well-established infection. 
Treatment of rats infected with Trypanosoma briuci b) 
1 lypanroth followed by bichloride of Mercury is superior to treatment 
by Trypanroth alone, but inferior to the combined treatment b) 
Atoxyl and bichloride of Mercury. 
IX. Many subinoculations were made during the work to ascertain 
whether the animals experimented with were infected. The great 
majority of subinoculations made from animals in whose blood 
trypanosomes could not be seen were negative; thirteen, made under 
apparently identical circumstances, were positive. An examination 
of these subinoculations is interesting. 
