325 
Similar experiments were carried out on two donkeys in order to 
obtain larger quantities of blood, so as to make a more detailed 
examination of the distribution of the arsenic with regard to the 
constituents of the blood. • • u u 
This table shows that the haemoglobin contained arsenic m both 
cases:— 
Table II 
Ssodium Arsenate 
Haemoglobin arsenic present arsenic present 
Stroma arsenic present arsenic absent 
Serum arsenic present arsenic absent 
The above recorded experiments confirm and extend the view 
that the amido group in Atoxyl and allied compounds in vitro, as 
well as in vivo, combines with the serum proteins. 
After the mode of the combination of Atoxyl and serum proteins 
in the animal organism had been established, it seemed necessary to 
estimate the amount of Atoxyl which is secreted in order to form an 
idea as to how much of the drug is actually left in the body. 
For this purpose a horse was injected subcutaneously with Atoyh 
and the urine and faeces analysed. The arsenic w^ estima 
according to Dupas-Giller's^ iodine method, using Gileass an 
Shearer’s modifiication. 
Table III gives the amount of Atoxyl injected and recovered m 
urine and faeces: — 
Table III 
Urine 
Date of Injection 
Amount Injected 
28.2.08 
I gm. Atoxyl 
2.3.08 
I gm. Atoxyl 
5 - 3-08 
I gm. Atoxyl 
7.3.08 
I gm. Atoxyl 
10.3.08 
2 gm. Atoxyl 
12.3.08 
2 gm. Atoxyl 
16.3.08 
2 gm. Atoxyl 
19.3.08 
2 gm. Atoxyl 
22.3.08 
I gm. Atoxyl 
Date of Collecting Arsenic Recovere 
1.3.08 
3.3.08 ^ 3 % 
6.3.08 78% 
8.3.08 S2% 
11.3.08 79 % 
13,3.08 ^ 5 % 
17,3.08 
20.3.08 
23.3.08 
