.384 
the infected animals from which the serum was obtained, and also to 
blood corpuscles of normal dogs or puppies. 
The serum was used both fresh and after being heated for 
thirty minutes at a temperature of 56° C.; and in this latter case with 
and without the addition of complement- (fresh centrifugalised dog 
serum). 
The blood corpuscles were usually washed three times in 0‘9% 
saline solution, and used in a 10% suspension. 
Serum of a dark reddish colour taken from a puppy three hours 
before death : haemoglobinuria marked. 
CA. C.C. C.C. C.U C, 
A. 10 per cent, suspension of 
infected dog’s red blood 
corpuscles ... ... 1 i i i i t 
Fresh normal dog’s serum 
(diluted I in 10) 
Infected dog’s inactivated 
serum 
Two hours at 37* C, 
B. 10 per cent, suspension of 
infected dog’s blood 
corpuscles 
Infected dog’s serum 
Two hours at 37* C. 
C. 10 per cent, suspension of 
infected dog’s blood 
corpuscles 
Normal serum 
Two hours at 37® C. 
IJ- 10 per cent, suspension 
of normal dog’s blood 
corpuscles 
normal dog’s serum 
(diluted I in 10) 
Inactivated infected doe’s 
serum ... 
Two hours at 37° C. 
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