I 
0-5 
I I I I 
O'I 0*05 O'OI 0 005 
o 
o 
o 
E. 10 per cent, suspension 
of normal dog’s blood 
corpuscles 
Infected dog’s serum 
Two hours at 37® C. 
Similar experiments were carried out with the serum of a larger 
dog, withdrawn during the early acute stage of the infection, when 
parasites were present in small numbers. The serum was of a light 
reddish colour. The results were entirely negative : in none of the 
test tubes was there any sign of liaemolysis. 
The series of experiments was repeated in the same way as m e 
first experiment, with the serum of a puppy; the serum of which w 
of a dark brownish mahogany colour ; and also in these experiments, 
I C.C. of the infected serum added to i c.c. of 10% infecte 00 
corpuscles, with both activated and inactivated serum, ca^se 
extremely slight haemolysis, whereas in no other tube was t ere y 
sign of haemolysis noticed. r j *.u 
The same experiment was repeated with the serum of a ^og three 
weeks after the first appearance of the parasites, an a so wi 
serum of a puppy at the commencement of the rise o 
In no case was there any haemolysis of either norma re 
corpuscles, or of the red corpuscles of the infected anima rom 
the serum was obtained. 
CONCLUSION 
These experiments tend to prove that the haemolysrs and 
haemoglobinuria in infections with Piroplasma cams is 
to an Isolysm nor to an Autolysin, but “'"^^^^ites 
disintegration of red blood corpuscles after the escape o 
from them. 
