THE ETIOLOGY OF RABIES. 
219 
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I the preparation of the virus for the ^inoculations are exceed- 
i ;1 y simple. After the death of an animal from rabies, the 
* nal cord is removed with the greatest care and suspended in a 
E ik about half a metre high. These flasks have rather large 
i uths, which are tightly plugged with sterilized cotton and the 
: toms are covered about one inch deep with caustic potash to 
; sorb all moisture from the air. The room in which the flasks 
placed and in which the virus is prepared for the inoculations 
lept constantly at a temperature of 200°. The lower the tem- 
•ature, the longer the virulence of the cords may be preserved. 
The virus used for the inoculations is always prepared fresh,and 
doing this a piece, a few millimetres in length, is first snipped 
from a cord that has the desired degree of virulence, as de¬ 
mined by the length of time that it has been suspended in the 
;ks. This piece is then rubbed up with a small quantity of 
rilized chicken bouillon in a small conical glass. There is no 
: xt proportion between the amounts of spinal marrow and bouillon 
id in preparation of the virus. The spinal marrow, after being 
i this manner brought partially into suspension in the bouillon? 
: »n settles to the bottom of the glass, and the comparatively 
ar fluid above is used for the inoculations. These are made in 
i! case of the human being by hypodermic injections into the 
lular tissue of the anterior abdominal wall. An ordinary 
avaz syringe is used for this purpose, and from half a syringc- 
to a syringeful of the bouillon is injected, in each case depend- 
\ upon the age of the person under treatment. The spinal 
Arrows that are used in These inoculations are always those 
Gained from rabbits which have died from rabies after a period 
incubation of seven days. 
These inoculations are not followed, either immediately or 
i notely, by any constitutional disturbance, so far as I was able 
determine, and they produce only a slight local tenderness— 
rcely more than that caused by a hypodermic injection of 
j 
ij ii-phine. 
The order of the inoculation, as followed now in the treatment 
the human subject, is practically the same as that described 
)ve for rendering dogs refractory to the disease. 
