456 
THOMAS BOWHILL. 
stomach, however, presented the most serious pathological lesions 
which we have yet seen, its walls being much thickened and ex 
tending into the cavity in deep folds over an inch high on thetof 
of these folds, and between them were numerous ulcerations, cov¬ 
ered with diptherdic masses of a yellowish color, and numerous) 
haemorrhagic centres were dispersed throughout; the same hac 
teria presented themselves again in this case. By your permis 
sion and hoping I am not imposing on your patience, I will now 
describe the method we employ to demonstrate the presence ol 
the bacteria in hardened specimens. It is by a system of double 
staining as follows : You can use either gentian violet or methylia 
violet, but for this organism I prefer the latter. Take a small 
porcelain dish, fill it half full of methyl violet and an equal quan¬ 
tity of caustic potash 1 to 10,000 of water; both of the above 
must be carefully filtered to guard against germs in the same. 
Take a very thin section of your tissue and put into the above 
mentioned dye for twenty-four hours. From this it goes into 
Gramm’s solution of iodine for five or ten minutes. The solution 
is prepared as follows: Distilled water one hundred per cent; 
iodine of potassium one per cent., and crystals of iodine one- 
half per cent. The above has the power of fixing the color in 
the germs. 
Now take the section and fade out until it acquires a light] 
blue color, in one per cent, hydrochloric acid alcohol, from this 
the section goes into sixty per cent, alcohol, and then into an 
aquious solution of eosin and leave for about one minute. Now 
put it into ninety per cent, alcohol, then into absolute alcohol, 
and lastly into oil of cedar for examination, aud you will find, in 
a properly colored specimen, that the tissues are colored red, and 
the bacteria, if present, of a deep azure blue with pale centers. 
In passing the section through this series of alcohols, great care) 
must be used not to leave them immersed too long, as by so do¬ 
ing you will destroy the appearance of the bacteria, and render 
their recognition even to an experienced eye an impossibility. 
In conclusion, there is one problem that is of great practical 
interest to the veterinary profession both in this country and 
abroad. That is, whether the disease in England, described by 
