84 SYNOPSIS OF CONTINENTAL VETERINARY JOURNALS. 
which we have examined, either at the infected locality or 
at Lyons, we have never been able to determine the presence 
of Bacteria. We only have found a more or less marked 
presence of corpuscles, refractive at their centres, but with 
dark peripheries, the nature of which we could only decide 
by culture or by inoculations. Besides, as we could not 
say absolutely that no Bacteria were present, it was necessary 
to prove this by inoculation or cultivation. Cultures in a 
moist chamber with aqueous humour and in Pasteur’s tubes 
with acid or alkaline human urine gave no Bacilli anthracis. 
As for our inoculations, numbering now thirty-four in all, 
they have been made by different methods, and on animals 
of various races and species, in order to neutralise the 
chance of any deficiency in receptive power of the organism, 
or of the animal inoculated. Sometimes inoculation was 
performed by incision through the skin with a lancet, some¬ 
times by hypodermic injection, and sometimes by injection 
into the veins. The animals inoculated were three young 
oxen, three sheep (one a cross-bred merino of Bassigny, the 
others of an Auvergne breed), two horses, and twenty-six 
rabbits and mice. All these gave negative results, not one 
of the animals succumbing to the effects of Bacillus anthracis . 
Hence we believe we are in a position to conclude that 
neither the blood nor the fluids from the swellings and the 
lymphatic glands of oxen affected with the disease known as 
‘ symptomatic charbon’ by Chabert contains the charbon 
bacterium nor its germs. We must, then, no longer con¬ 
sider this affection as a superficial and local indication of 
systemic anthrax, and we must no longer think that the 
tumour indicates the seat of entry of the anthrax Bacillus. 
In the true malignant pustule of man we always find Bacilli 
present in abundance. These experiments acquire the more 
importance, since simultaneously nineteen inoculations were 
made with blood from animals which, in the same neigh¬ 
bourhood, had died of splenic fever. These invariably led 
to the death of the subject. Simultaneously M. Thomas 
made parallel experiments with perfectly fresh fluids—in all 
cases negative. Having proved thus much, M. Arloing 
and his collaborators express a hope of some day being in a 
position to determine whether there is any Bacterial 
organism characteristic of this affection.” The Becueil of 
December last gives us some further particulars with regard 
to the discussion which took place at the Academy des 
Sciences on the 4th of November between M. Colin and M. 
Pasteur. The former read a very long memoire , “On the 
Persistence of Virulent Properties in Carcases and Cadaveric 
