792 
BACILLUS ANTIIRACIS. 
of the Interior, and the result was remarkable, for in 1879 
only 157 cases of pleuro-pneumonia occurred in the whole 
country. That, he thought, was an encouragement to them 
to proceed in the course upon which they had embarked, and 
he ventured to hope that the time was coming when stock- 
owners in this country, feeling secure about the health of 
their cattle would go on breeding, and that wfith their improved 
breeds and other advantages they possessed over the foreigner, 
they would be able to compete with everybody all over the 
globe .—Mark Lane Express. 
MODIFICATION OF THE PROPERTIES OF BACILLUS 
ANTHRACIS BY CULTIVATION. 
In the course of some experimental investigations into the 
pathology of anthrax at the Brown Institution, made during 
the past twelve months, two series of phenomena have been 
the subject of study, and in each some results have been 
attained w hich Professor W. S. Greenfield (in a “ preliminary 
note ”) believes to be novel, and of considerable practical 
importance if verified by other observers. 
The practical purpose of these investigations was to ascer¬ 
tain (1) by what means the virus of splenic fever may be so 
modified as to be capable of inoculation without fatal result, 
and (2) whether a modified attack, produced by inoculation, 
exerts any protective influence against a future inoculation 
with unmodified virus. 
The conclusions arrived at by these experiments were as 
follows : 
1. That anthrax may be artificially communicated to bovine 
animals by inoculation with the blood or spleen of the 
guinea-pig w 7 hich has died of the disease artifically induced, 
and that the same result may be attained by inoculation 
with the Bacillus anthracis cultivated from the fluids of a 
rodent; the disease thus induced being severe, but rarely 
fatal, to previously healthy bovine animals, a result previously 
attained by Dr. Burdon-Sanderson independently. 
2. In all the cases thus inoculated, the animals appeared 
to have acquired either a considerable degree of protection or 
entire immunity from the results of subsequent inoculation, 
although much larger doses of the virus w 7 ere employed. 
In the course of these experiments the author employed 
on several occasions Bacillus anthracis artifically cultivated in 
