184 
Journal of Agricultural Research 
Vol. XXIII, No. 3 
animal was found dead the morning of the fourth day after treatment, 
the larger animal being killed by us the sixth day to conclude the experi¬ 
ment. The results contrast very strikingly with the deaths in 36 hours 
of mature animals given much smaller doses. No plausible explanation 
occurs to us in this connection, but it is possible that further work along 
this line will throw some light on the subject. 
As regards efficacy, it will be seen that the drug as given in our cases 
was 100 per cent effective against stomach worms in the case of both 
animals; 48 per cent effective against hookworms in the case of the first 
animal and 99 per cent in the case of the second animal, regarding the 
two hookworms found in the cecum as removed by the drug; 100 per cent 
effective against nodular worms in the case of both animals; 100 per cent 
effective against whipworms in the case of the first animal, the only one 
infested; and o per cent effective against tapeworms in the case of the 
first animal, the only one infested. The drug was therefore extremely 
effective in removing stomach worms, nodular worms, and whipworms; 
it removed from almost half to almost all the hookworms present, but was 
of no value in removing tapeworms. These results warrant further 
investigations to determine the actual utility of this drug in treating 
cattle infested with stomach worms, nodular worms, hookworms, and 
whipworms to determine whether it can be given in a dose which will be 
effective without injury to the animal treated. 
TESTS ON MONKEYS 
In the experiments on monkeys, carried on in cooperation with Dr. 
G. C. Lake, of the Public Health Service, we gave doses of twice to five 
times the indicated human dose of 3 cc., the latter based on the fact that 
the dose for the adult person and the average-sized dog is usually the 
same for anthelmintics and many other drugs. These monkeys weighed 
from 4.5 to 7.5 pounds. All monkeys survived the dose given, one of 
them weighing 4.5 pounds receiving 12 cc., or approximately 6 cc. per 
kilo. Taking the weight of an average-sized man as 70 kilos, and assum¬ 
ing that man could tolerate this drug at the same rate, the equivalent 
dose for the man would be about 420 cc. At this rate the indicated 
therapeutic dose of 3 cc. would have a safety factor of 140. While it is 
not assumed that this is true, nevertheless it appears that this drug 
should prove quite safe for man. Its complete efficacy against the blood¬ 
sucking worms, hookworms in dogs, Strongylus in horses, and stomach 
worms in sheep indicates that it will probably prove equally effective 
against hookworms in man. 
The monkeys in this experiment were kept under observation for one 
month and then were released from this experiment. An attempt was 
made to determine something of the anthelmintic value of the drug in 
the case of monkeys, but owing to a misunderstanding the attendant 
did not collect the feces of the first 24 hours after treatment. The feces 
for the next 48 hours contained 1 whipworm and 34 heterakids, appar¬ 
ently Subulura distans. The drug is evidently of some value in removing 
heterakids from monkeys, but owing to the fact that no post-mortem 
examination was made and the doses used were very high, little can be 
concluded as to its value. 
In a recent paper, Lake (2 5) has published the results of some further 
tests on monkeys. He finds that monkeys weighing 2.21 to 2.63 kilos 
tolerate 12 to 16 doses of 1, 2, 3, and 5 cc. each at intervals of 2 to 3 days 
