368 
Journal of Agrtculiural Research 
Vol. XXIII, No. 5 
No growth occurred in any of the cultures in the jar. The bouillon 
control in the room showed good growth. The methylene blue had faded 
considerably in n days. The litmus did not fade until 15 days after 
setting up the experiment. A careful watch was kept for over 3 weeks, 
but no growth took place in the cultures until they were removed from 
the jar. (Tike the Lewis organism.) 
RELATION TO MOISTURE 
This test was followed out according to Mr. Lewis's rod method. Glass 
rods were held in place in test tubes by passing them through the cotton 
plugs, after which the tubes were sterilized. Next the rods were dipped 
to a uniform depth in a 6-day-old bouillon culture, returned to the tubes, 
and left to dry at room temperature which varied from 18 0 to 23 0 C. 
Care was taken that the rod did not rest against the side of the tube and 
prevent a uniform drying. At intervals of 24 hours several of these rods 
were transferred each to a tube of beef bouillon. Growth occurred in one 
test after the cultures on rods had been dried 7 days. In a second test 
made exactly the same way, growth did not occur when dried 7 days but 
did occur at 6 days. Evidently 6 days is about the limit of drying for 
a 6-day-old culture of this organism. (Like Lewis's organism.) 
GROWTH IN FERMENTATION TUBES 
Gas formation. —The organism is aerobic so far as tested and does 
not form gas. It was tested in fermentation tubes in the presence of 
each of the following carbon compounds: saccharose, dextrose, lactose, 
maltose, and glycerin; 1 per cent of these being added to a 2 per cent 
water solution of Witte's peptone. No gas formed in any of the tubes. 
Growth occurred in the open arm of each tube but none in the closed arm. 
The cultures were tested for acidity after they had grown 21 days. 
Five tests were made—two in January, in which Witte's peptone was 
used in one and Difco peptone was used in the other. Another test 
containing Witte's peptone was made in June. The fourth and fifth 
tests were made in July with Witte's and Difco peptone, as shown by 
Table I. Tests 1,2, and 3 are with Witte’s peptone, 4 and 5 with Difco. 
The results are as indicated in Table I, and it is interesting to note how 
the same organism varies. With Difco peptone, the acidity of the 
medium was considerably reduced by the growing organism. 
Titrations with phenolphthalein were made before inoculating and 
again after the organism had grown 21 days. The first test after 21 days 
showed there was little change in acidity in the cultures. The saccharose, 
dextrose, and glycerin cultures were slightly more acid than the controls; 
the maltose and lactose cultures were less acid. In the second test after 
21 days all cultures were more acid than the controls. In the third test 
after 21 days there was no change in acidity with dextrose, lactose, and 
glycerin; there was increase with saccharose and decrease with maltose. 
The fourth and fifth tests after 21 days were made with Difco peptone. 
Titrations showed there was decidely less acidity in the cultures than in 
the uninoculated media. 
To determine further the changes in acidity, some of each of the same 
medium was tested with the indicators brom thymol blue, brom cresol 
purple, phenol red or cresol red, before inoculating. This was done at 
the same time they were tested on Fuller's scale. In all five tests the 
