6 4 S 
Journal of Agricultural Research 
Vol. XXIII, No. 8 
Tabi,e II .—Effect offormaldehyde on mycelium 1 
Treatment. 
Num¬ 
ber 
viable 
in con¬ 
trol, un¬ 
treated. 
Number viable after treatment. 
3 
mm. 
5 
nun. 
8 
min, 
zo 
min. 
i.S 
mm. 
20 
min, 
30 
mm. 
1. Formaldehyde, 1-50. 
9 
9 
8 
7 
2 
0 
O 
O 
2. Formaldehyde, 1-50. 
0 
1 
O 
0 
Q 
3. Formaldehyde, 1-50. 
7 
0 
0 
O 
0 
O 
4. Formaldehyde, 1-100. 
y 
9 
8 
8 
4 
5 
0 
O 
0 
5. Formaldehyde, 1-100. 
Q 
0 
0 
0 
O 
6. Formaldehyde, 1-100. 
7 
0 
y 
A 
0 
0 
O 
Q 
7. Formaldehyde, 1-200. 
7 
0 
V 
0 
0 
A 
O 
O 
8. Formaldehyde, 1-200. 
y 
9 
7 
9 
y 
9 
7 
6 
O 
1 The mycelium of treatments i and a was taken from diseased lettuce plants; all others from the surface 
of 8-day-old agar cultures. Nine lots of mycelium used in each case. 
EFFECT OF ASPIRATING SCLEROTIA ON SUBSEQUENT TREATMENT WITH 
FORMALDEHYDE 
To test the effect which surface and intercellular air might have on 
the action of formaldehyde on sclerotia, a number of sclerotia were aspi¬ 
rated in water in an aspirating bottle for l /2 hour. Subsequently they 
were treated with a 1-100 formaldehyde solution, washed aseptically, 
and plated on nutrient agar. A comparison with the untreated controls 
shows that the process failed to shorten the time in which the sclerotia 
were killed. (Table III, treatments 6 and 7.) 
