33 
error  of  experiment.  Moreover,  such  diurnal  variations  in  health 
have  their  counterpart  in  the  much  larger  variations  in  disease,  as 
will  be  seen  later  in  Table  24.  Here  we  have  evidence  of  variation 
in  the  content  of  haemoglobin  per  unit  volume  of  wet  red  blood  cells 
which  is  capable  of  independent  variation  in  health.  When,  however, 
the  variations  in  haemoglobin  in  different  samples  of  blood  from  the 
same  individual  in  Table  16  are  compared  with  corresponding  values 
of  K,  it  is  seen  that  no  constant  relationship  between  the  two  is 
recognisable.  The  mean  value  of  K  at  37°  C.  for  the  Y  series  is 
0‘003S,  and  for  the  B  series  0^0030. 
In  monomolecular  reaction  rates  at  different  temperatures  the 
relation 
holds,  Ti  and  being  the  absolute  temperatures  at  which  two  estima¬ 
tions  of  K  are  carried  out.  To  ascertain  if  this  relation  held  for  the 
haemolysis  of  red  cells  by  quinine,  the  series  of  experiments  recorded 
in  Table  17  were  carried  out  at  temperatures  of  37°  C.,  31°  C.  and 
25°  C.  respectively,  the  concentration  of  quinine  (in  the  alkaloidal 
state)  being  0  001 1 3  M.  (0’0366  per  cent.).  In  order  to  avoid  the 
effect  of  the  diurnal  variations  in  the  composition  of  the  red  cells, 
illustrated  in  Table  16,  each  of  the  three  sets  of  determinations 
(Experiments  i  to  4,  Table  17)  was  carried  at  the  same  time  with 
portions  of  the  same  emulsion  of  red  cells  in  quinine  solution.  In 
Table  18,  with  the  aid  of  the  values  of  K  obtained  from  Table  I7i 
the  value  of  was  determined.  Before  proceeding  to  consider  the 
value  of  the  latter  constant,  however,  a  few  remarks  may  be  made 
upon  the  data  obtained  in  the  former  Table. 
In  the  first  place,  it  is  to  be  observed  that  the  same  initial  delay  and 
terminal  acceleration  of  the  process  of  haemolysis,  which  has  already 
been  noted  at  37°  C.  in  Table  16,  occurs  also  at  31°  C.  and  25°  C. 
When  the  concentration  of  the  red  cells  has  been  reduced  by 
haemolysis  to  about  80  per  cent.,  the  reaction  proceeds  at  all  three 
temperatures  at  a  tolerably  constant  rate  until  the  concentration  of  red 
cells  reaches  about  20  per  cent.  Between  these  limits  the  values  of  K, 
calculated  for  a  monomolecular  rate,  do  not  exhibit  any  very  great 
variation ;  when  calculated  for  a  bimolecular  or  multimolecular 
reaction  rate,  the  variations  obtained  are  sufficiently  great  to  exclude 
c 
