40 
recorded,  with  an  experimental  error  of  —  2’6  per  cent,  and  —  r5  per 
cent,  respectively. 
The  observations  recorded  in  Tables  20  and  21  shov/  that  under 
the  conditions  of  experiment  obtaining,  which,  as  already  mentioned, 
were  similar  to  those  in  Table  i,  namely  with  a  quinine  sulphate 
solution  of  about  0^05  per  cent,  concentration  and  with  an  amount  of 
Table  20.  Determination  of  the  amount  of  quinine  withdrawn  from  solution  by  healthy 
human  red  blood  cells.  Duration  of  action  of  red  blood  cells  on  quinine  sulphate 
dissolved  in  o'g  per  cent,  solution  of  sodium  chloride  eighteen  to  twenty-four 
hours.  Temperature  of  experiment,  17°  C.  to  19°  C. 
No.  of 
Experi¬ 
ment 
Amount  of 
quinine 
sulphate 
taken 
Equivalent 
amount  of 
quinine 
Concen¬ 
tration  of 
quinine 
sulphate 
Amount 
of  quinine 
recovered 
Percentage 
of  quinine 
recovered 
Weight  of 
wet  red 
cells  taken 
Weight  of 
wet  red 
cells  : 
Weight  of 
quinine 
sulphate 
I 
0*1263 
0-1098  g. 
0-0473  g. 
0*1050  g. 
95-6% 
1-5  g* 
12  ;  I 
2 
0-1263  s- 
o-iofl8  g. 
0-0547  g. 
0-1047  g- 
95'4% 
1-8  g. 
14  :  I 
3 
0*1263 
0-1098  g. 
0*0522  g. 
0*1071  g. 
97- .3  % 
2-2  g. 
17  ;  I 
4 
o-Ilq3  g. 
0-1037  g- 
0-0529  g. 
0-1023  g- 
98*8  % 
2-6  g. 
22  :  I 
5 
0*1263  8- 
0-1098  g. 
0-0541  g. 
0-0985  g. 
88-7% 
7'9  g- 
63  ;  I 
6 
0-1193  g. 
0-1037  g- 
0-0534  g. 
0-0907  g. 
87-7% 
10-7  g. 
90  :  I 
7 
0*1 189  g. 
0-1032  g. 
0-0571  g. 
0-0808  g. 
78-3% 
12-0  g. 
loi  :  I 
8 
control 
o-r267  g. 
o*  r  roo  g. 
0-0517  g. 
0*1071  g. 
97'4  % 
— 
— 
9 
control 
0*1267  S' 
o*  1 1 00  g. 
0-05 1 7  g. 
0-1084  g. 
98-5% 
red  blood  cells  in  such  excess  as  to  prevent  any  trace  of  haemolysis 
during  the  course  of  the  experiment,  quinine  is  withdrawn  from 
solution  by  red  cells.  In  the  first  four  experiments  the  withdrawal 
of  quinine  from  solution  by  the  action  of  the  red  cells  is  not  satis¬ 
factorily  exhibited  since  the  amount  taken  up  lies  too  near  the  error 
of  experiment.  When,  however,  the  weight  of  red  cells,  instead  of 
being  I’S  to  2'6g.  [Experiments  i  to  4,  Table  20]  is  from  7'9g- 
to  1 2  g.,  then  the  amount  of  quinine  withdrawn,  though  relatively 
small,  lies  altogether  beyond  the  range  of  experimental  error  and 
becomes  readily  ascertainable,  varying  between  1 1  per  cent,  and 
22  per  cent,  of  the  amount  originally  present  (Table  23).  It  is  thus 
clear  that  the  red  cells  take  up  quinine,  and  the  amount  which  is 
combined  is  found  (Experiments  5,  6  and  7)  to  be  about  o' 14  per 
cent.  If  we  assume  that  the  amount  of  quinine  taken  up  by  varying 
amounts  of  red  cells,  when  the  final  concentration  of  quinine  in  the 
