41 
surrounding  fluid  is  in  all  cases  the  same,  is  proportional  to  the  weight 
of  red  cells  present,  then  in  a  final  concentration  of  0'0404  per  cent, 
the  amount  of  quinine  taken  up  by  2  g.  of  wet  red  cells  would  be 
about  3  per  cent,  of  the  amount  originally  present  in  solution,  under 
the  conditions  of  experiment  obtaining  in  Table  20.  In  view  of  the 
results  obtained  in  the  comparative  experiments  recorded  in  Table  g, 
it  may  be  doubted  if  this  simple  relation  holds.  It  may  be  suggested 
that  the  quantity  taken  up  by  the  smaller  amounts  of  red  cells  would 
Table  21.  Determination  of  the  percentage  amount  of  quinine,  contained  in  red  blood 
cells  in  the  experiments  recorded  in  Table  20. 
No.  of 
Experi¬ 
ment 
Concentration  of  quinine  sul¬ 
phate  originally  present 
Weight  of 
wet  red  cells : 
Weight  of 
quinine 
present  at 
beginning 
of  experi¬ 
ment 
Percentage 
of  the 
quinine, 
originally 
present, 
which  was 
withdrawn 
from  solution 
by  red  ceils 
Percentage 
of  quinine 
in  wet  red 
cells 
Final  con¬ 
centration  of 
quinine  in 
liquid  part 
of  emulsion 
of  red  cells 
r 
0*04.7'?  =  0*0412%  quinine 
13-7  ;  I 
(4-4%) 
(0-320%) 
0-0393  % 
2 
0-0547  %  =  °'°47i;  %  » 
16-4  :  1 
(4-6%) 
(0-284  %) 
0-0453  % 
3 
0-0522  %  =  0-0453  %  „ 
20*0  :  I 
(2-5  %) 
(0-123%) 
0-0442  % 
4 
0-0529  %  =  0-0460  %  „ 
25-0  :  I 
(■•^%) 
(0-054%) 
0-0455  % 
5 
0-054I  %  =  0-0470  %  „ 
89-7  :  t 
”•3% 
0-143% 
o-cM.17% 
6 
o-0534%  =  0-0464%  „ 
103-3  :  ' 
12-3% 
0-121  % 
0-0407  % 
7 
0-0571%  =0-0496%  „ 
116-3  !  I 
21-7% 
0-187% 
0-0388  % 
mean  of 
5,  6  and  7  =  0-0477  quinine 
mean  of  5,  6 
and  7  = 
0-137% 
mean  of  5,  6 
and  7  = 
0-0404 
probably  be  relatively  greater  than  that  taken  up  by  the  larger 
amounts  in  Tables  20  and  21.  Unfortunately  the  determination  of 
the  amount  taken  up  by  small  quantities  of  red  cells,  representing 
the  small  difference  of  two  estimations,  each  subject  to  an  error  of 
experiment,  which  is  not  of  negligible  dimensions,  cannot  be  carried 
out  with  sufficient  accuracy  to  settle  this  point. 
Returning  now  to  the  enquiry,  which  formed  the  starting  point 
-of  the  experiments  m  Tables  20  and  21,  namely,  whether  quinine  is 
used  up  to  any  considerable  extent  when  acting  upon  red  cells  so  as 
to  cause  haemolysis,  we  find  that  under  the  conditions  obtaining  in 
the  experiments  recorded  in  Table  i  the  concentration  of  the  cjuinine 
solution  undergoes  a  diminution,  but  that  the  extent  of  the  diminution 
is  very  small.  The  conclusion  arrived  at  on  p.  38  is  therefore 
confirmed. 
