I8 
Up  to  the  present  only  waished  red  blood  cells,  freed  Irom  plasma, 
have  been  considered.  In  Table  2,  however,  side  by  side  with  the 
results  obtained  with  washed  red  cells,  are  given,  in  brackets,  those 
obtained  with  the  same  amounts  of  red  cells  not  freed  from  plasma. 
The  haemolysis  in  the  latter  case  is  not  so  marked  as  in  the  former, 
the  transition  point  being  reached  when  a  concentration  of  quinine 
bihydrochloride  amounting  to  O’ 188  per  cent,  is  attained  (as  against 
0’I55  per  cent,  in  the  former  series),  and  the  weight  of  red  blood  cells 
haemolysed  is  thirteen  times  that  of  the  quinine  salt  present  (as 
against  i  S'6  in  the  former  series),  some  of  the  quinine  being 
apparently  taken  up  by  the  plasma  and  thus  prevented  from  acting 
upon  the  red  blood  cells.  There  is  also  noticeable,  less  regularity  in 
this  series,  when  compared  with  the  former.  This  is  perhaps  attri¬ 
butable  to  diurnal  variations  in  the  composition  of  the  plasma. 
In  Table  4  the  action  of  quinine  bihydrochloride  upon  red  blood 
cells  contained  in  more  or  less  diluted  oxalated  plasma  is  shown.  In 
Table  4.  Haemolysis  of  red  blood  cells  by  quinine  bihydrocholoride  dissolved  in  the 
blood  plasma.  Temperature  of  experiment  37®  C. 
Composition  of  Mixture  of  Oxalated 
Blood  and  Solution  of  Quininf. 
No.  of 
Source 
Bl  HYDROCHLORIDE 
Kxperi- 
of  blood 
ment 
blood 
Q.2IICI 
weight  of  wet  red  celh 
weight  of  Q.2HCI 
I 
Human 
96% 
0-21 
195  :  I 
92  “1. 
0-41  % 
c/, 
I 
3 
9°  % 
®  /o 
77 
r 
4 
89?'q 
0-54% 
71 
I 
h 
88  % 
°’57% 
67 
I 
6 
88% 
O’ 58% 
64 
1 
7 
,, 
87% 
0-62 
60 
I 
s 
•? 
87% 
0*62  % 
60 
I 
9 
84% 
0-8 1  % 
45 
I 
:o 
5°  % 
O’ 5. 3  % 
4^ 
r 
1 1 
Rabbit 
4Ub 
0'96% 
17  :  1 
Condition  of  mixture  at  end  of 
experiment 
Plasma  contained  0*6  %  of  dissolved 
haemoglobin 
at  the  end  of 
three  hours. 
U  *^*4  A)  J’ 
5)  0*8  j, 
V  1-8%  „ 
"  -'5%  ’! 
V  3’I% 
3  7.0  •} 
6-8  %  „ 
2'’*  r 
'3  h  .’U  M 
Red  cells  completely  haemolysed  at 
end  of  7  hours. 
Red  cells  completely  haemolysed  at 
end  of  15  mins. 
these  experiments  the  blood  employed  formed  from  42  per  cent,  to 
96  per  cent,  of  the  mixture.  As  in  the  preceding  experiments  the 
protective  action  of  the  plasma  is  well  marked.  Thus  in  Experiment 
9,  in  which  84  per  cent,  of  blood  is  present  and  the  concentration  of 
