1.5 
of  the  period  of  experiment,  namely,  three  hours,  the  temperature  of 
experiment  being  37°  C.  In  Table  i,  only  the  concentration  of 
quinine  salt  and  the  ratio  of  the  weight  of  wet  red  cells  to  the  weight 
Table  i.  Haemolysis  of  red  blood  cells  by  quinine  bihydrochloride  dissolved  in  0*9  per 
cent.  NaCl  solution.  Duration  of  experiment  three  hours.  Temperature  C. 
No.  of 
Experi¬ 
ment  . 
Composition  of  Mixture  of 
Red  Blood 
Cells  and  Quinine  Solution 
Quinine  bihydrochloride  0*080  % 
Weight  of  wet  red  blood  cells  c;*! 
Weight  of  quinine  salt  i 
0*065  % 
6-5' 
0'054% 
°-o45  % 
10-4 
I 
0-038  % 
'37 
1 
I 
Complete 
Complete 
Complete 
Complete 
Partial 
2 
Complete 
Complete 
Complete 
Complete 
Market! 
3 
Complete 
Complete 
Complete 
Complete 
Partial 
4 
Complete 
Complete 
Complete 
Complete 
Partial 
5 
Complete 
Complete 
Complete 
Complete 
Partial 
of  quinine  present  are  given,  the  actual  quantities  of  red  blood  cell 
emulsion,  quinine  solution  and  of  salt  solution,  which  were  employed, 
being  omitted  so  as  not  to  overburden  the  Table  with  such  details 
of  experiment  as  are  best  arranged  in  accordance  with  the  actual 
conditions  of  working  which  individual  experimenters  may  regard  as 
most  convenient.  In  some  of  the  later  experiments,  however,  the 
concentration  of  the  solutions  employed  and  the  amounts  of  each 
used  are  given  (Table  24) ;  from  these  the  reader  will  readily  devise 
convenient  concentrations  and  quantities  to  employ  in  those 
experiments  in  which  these  details  are  omitted. 
During  the  course  of  the  e.xperiments  the  tubes  containing  the 
mixtures  of  red  blood  cell  emulsion  and  quinine  solution  were  stirred 
with  a  glass  rod  every  fifteen  minutes.  In  the  tubes  in  which 
complete  haemolysis  appeared  to  have  occurred,  a  microscopical 
examination  of  the  deposit  after  centrifugalisation  was  made  in  order 
to  make  certain  that  this  was  the  case. 
In  all  these  experiments  the  glass  tubes  and  pipettes  used  were 
kept  scrupulously  clean.  From  time  to  time  a  search  for  bacteria 
was  made  at  the  close  of  the  experiment,  but  uniformly  with  negative 
result.  Actual  sterilisation  of  the  glass  apparatus  and  of  the  solutions 
employed  was  carried  out  only  in  a  few  cases  in  which  the  time 
elapsing  between  the  collection  of  blood  and  the  termination  of  the 
experiment  exceeded  four  hours. 
