^'5 
Table  29.  Experiments  made  to  ascertain  if  a  haemolytic  action  on  red  blood  cells  could  be  obtained  with 
the  blood  plasma  of  individuals  suffering  from  blackwater  fever. 
No.  of 
Obser¬ 
vation 
Case 
Condition  of  subject 
Ratio  of 
oxalated 
plasma  to 
-  5  /q 
emulsion 
of  red  cells 
Mixture 
kept  at 
For 
Then 
kept  at 
37°  c. 
for 
Result 
I 
2 
Sixteen  hours  before  attack  of  black- 
water 
20  :  r 
o°C.-+°C. 
30  min. 
3  hours 
No  isolysin  present 
in  blood  plasma 
2 
Two  hours  before  attack  of  blackwater 
During  attack  of  blackwater  ... 
,, 
4 
At  end  of  attack  of  blackwater 
I' 
15 
55  15 
5 
n 
Six  days  later  ... 
51 
51 
15 
6 
3 
'Fwenty  hours  before  attack  of  haemo- 
globinuria 
15 
No  autolysin  present 
in  blood  plasma 
15 
7 
55 
During  attack  of  haemoglobinuria  ... 
•5 
15 
S 
During  attack  of  haemoglobinuria  ... 
,, 
5' 
M 
9 
n 
At  end  of  attack  of  haemoglobinuria... 
,, 
1* 
10 
4 
'Fhree  days  after  attack  of  haemo¬ 
globinuria 
*5 
55  a. 
i  I 
5J 
Two  days  after  relapse  of  haemo¬ 
globinuria 
55 
5’ 
11  15 
12 
s 
Twelve  hours  after  disappearance  of 
haemoglobinuria 
55 
5* 
5’  55 
‘3 
6 
At  end  of  attack  of  haemoglobinuria 
55 
No  isolysin  present 
in  blood  plasma 
14 
6a 
During  attack  of  blackwater  ... 
55 
55 
55 
No  autolysin  present 
in  blood  plasma 
7 
During  attack  of  blackwater . 
„ 
5>  '5 
16 
7« 
During  attack  of  haemoglobinuria  ... 
■7 
8 
During  attack  of  haemoglobinuria  . . . 
18 
9 
Four  days  after  attack  of  haemo¬ 
globinuria 
'I 
*5 
Complete  haemo¬ 
lysis  of  red  cells* 
‘9 
?? 
Two  months  later 
55 
,, 
55 
No  autolysin  present 
in  blood  plasma 
20 
10 
During  attack  of  haemoglobinuria  ... 
,, 
21 
5) 
During  relapse  of  haemoglobinuria  ... 
22 
1  I 
During  attack  of  haemoglobinuria  ... 
23 
-A,t  end  of  attack  of  haemoglobinuria 
(during  suppression) 
55 
5* 
5' 
55 
24 
» 
Three  days  later  (during  suppression] 
25 
12 
At  end  of  attack  of  haemoglobinuria 
" 
55 
>' 
Haemolysis  of  red 
cellsf 
26 
One  day  later  .’. . 
-• 
5> 
No  autolysin  present 
in  blood  plasma 
27 
14 
At  end  of  attack  of  liaemoglobinuria 
,, 
55 
28 
'5 
During  attack  of  haemoglobinuria  ... 
29 
During  attack  of  haemoglobinuria 
(during  partial  suppression) 
n 
■’ 
'■ 
n 
30 
16 
At  end  of  attack  of  haemoglobinuria 
5« 
” 
55 
*5  55 
No  turbidity,  smell  or  other  indication  of  putrefaction.  Blood  plasma  examined  ten  hours  after  collection. 
Room  temperature  28*5°  C. 
t  Bacteria  present  in  blood  plasma,  which  was  examined  tw'enty-four  hours  after  collection. 
E 
