66 
In  1  able  2g  the  result  of  testing,  by  the  method  above  described, 
the  plasma  m  sixteen  attacks  of  blackwater  fever,  before,  during  and 
shortly  after  the  paroxysm,  is  given,  the  examination  being  usually 
made  within  an  hour  of  the  withdrawal  of  blood.  In  addition,  twenty- 
eight  other  observations  upon  healthy  individuals,  and  also,  for  the 
sake  of  comparison,  upon  ten  cases  of  malaria,  are  recorded  in  Table 
30. 
Table  30.  Experiments  made  to  ascertain  if  a  h-ieniolylic  action  on  red  blood  cells  could 
be  obtained  with  the  blood  plasma  of  individuals  suffering  from  malarial  and  other 
affections.  Normal  plasma  Is  also  employed  in  eleven  observations. 
Ratio 
of  oxa- 
lated 
plasma 
to  2-5% 
emulsion 
of  red 
cells 
Mixture 
kept  at 
For 
Then  kept 
at  37°C. 
for 
.  20  :  1 
o'‘C.-4°C. 
30  min. 
3  hrs. 
11 
11 
JJ 
•  M 
• 
i> 
1’ 
J) 
*  jj 
J) 
11 
11 
J1 
11 
11 
?3 
11 
41 
'1 
n 
11 
1J 
11 
11 
•> 
JJ 
” 
11 
51 
4J 
31 
11 
1) 
JJ 
1) 
11 
„ 
JJ 
4? 
4' 
11 
51 
>1 
1) 
n 
11 
1' 
11 
JJ 
11 
1? 
J) 
11 
n 
J4 
?i 
11 
11 
TJ 
JJ 
1’ 
11 
11 
JJ 
51 
11 
11 
JJ 
11 
>1 
J) 
JJ 
11 
JJ 
11 
ji 
11 
11 
11 
JJ 
11 
11 
11 
JJ 
11 
’1 
J1 
J) 
11 
11 
11 
J) 
No.  of 
Obser¬ 
vation 
Condition  of  subject 
1 
Healthy  ... 
2 
,,  4  days  later 
3 
,,  10  days  late 
4 
Healthy 
5 
„  2  days  later 
6 
„  5  days  later 
7 
Healthy  . 
8 
Healthy 
9 
Healthy  (Native) 
10 
Healthy  ... 
t  I 
Healthy  ... 
12 
Typhoid  ... 
13 
Pyrexia  (Native)  ... 
14 
Pleurisy  (Native) 
‘5 
Bullous  eruption  on 
16 
and  legs  (Native) 
Pneumonia  (Native) 
17 
Malaise  ("Native)  ... 
18 
Malaria 
'9 
Uraemia,  T.  106°  F. 
20 
Malaria 
21 
,,  next  day  ... 
Result 
22 
23 
24 
25 
26 
27 
28 
Malaria,  T.  104°  F. 
Malaria  (Indian)  ... 
bilious  fever 
Malaria 
Malaria 
Malaria 
Malaria 
No  autolysin  present 
in  blood  plasma 
No  isolysin  present 
in  blood  plaitma 
■K  • 
No  autolysin  prest 
in  blood  plas: 
No  isolysin  presi 
in  blood  plasi 
No  autolysin  present 
in  blood  plasma 
No  isolysin  present 
in  blood  plasma 
Slight  haemolysis  of 
red  cells  * 
No  isolysin  present 
in  blood  plasma 
Partial  clotting  of  blood  occurred  during  collection. 
