77 
urine  was  also  tested  for  coagulable  proteid,  any  deposit  ]-)i-esent  being 
submitted  to  microscopical  examination.  In  Experiments  8,  Q  and 
10  the  urine  was  collected  continuous!}'  by  means  of  a  cannula 
introduced  into  the  bladder,  in  the  other  experiments  it  was  voided 
naturally. 
Before  experiment,  the  o.xalated  blood  plasma  of  the  rabbits 
employed  was  found  to  be  of  a  very  faint  yellow  tint,  almost  colourless. 
On  spectroscopic  examination  no  haemoglobin  bands  were  as  a  rule 
seen,  though  on  one  occasion  0'05  per  cent,  of  dissolved  haemoglobin 
was  met  with.  The  weight  of  the  rabbits  ranged  from  912  g.  to 
1,760  g.,  the  total  amount  of  blood  the  animals  possessed  varied  from 
75  c.cm.  to  1 18  c.cm.,  and  the  ratio  of  blood  to  body  weight  (Table  35) 
was  ytt  to  the  higher  fraction  being  reached  in  the  younger,  and 
therefore  lighter,  animals. 
The  amount  of  haemoglobin  injected  was  that  obtainable  from 
0'44g.  to  7'5^g-  of  cells  (m  the  moist  condition).  In  all  except 
Experiments  6  and  8,  Tables  35  and  37,  the  haemoglobin  was 
obtained  from  the  animal’s  ow'n  red  cells.  The  amount  required  in 
the  two  latter  experiments  being  too  large  to  be  conveniently  taken 
from  the  animals  subjected  to  experiment,  part  of  the  haemoglobin 
injected  was  obtained  from  other  rabbits.  The  injection  of  laked 
blood  produced  no  recognisable  ill  effect,  the  animals  taking  food 
well  during  the  experiment  and  seeming  in  every  respect  normal.  In 
Experiment  5  the  animal  was  apparently  unaffected  by  the  injection, 
but  died  suddenty  at  the  end  of  eight}^-five  minutes ;  possibly  in  this 
case  death  wms  due  to  embolism,  for  the  stromata  of  the  laked  red 
cells  were  not  completely  removed  from  the  haemoglobin  solution 
before  injection. 
After  the  injection  of  dissolved  haemoglobin  into  the  vein  of  the 
ear,  the  blood  plasma  of  the  injected  animal  became  of  a  reddish 
colour.  At  the  end  of  three  to  twenty  minutes,  estimations  of  the 
amount  of  haemoglobin  present  showmd  that  this  ranged  from  078  per 
cent,  to  iO'8o  per  cent.  The  amount  present  immediately  after 
injection  may  be  calculated  in  the  manner  indicated  on  p.  go,  and 
is  found  to  be  somewhat  higher,  ranging  between  0'8i  per  cent,  and 
12' 1 5  per  cent.  As  the  amount  of  haemoglobin  injected  intravenously 
was  known,  the  total  volume  of  the  plasma  of  the  rabbit  could  be 
