sonicwliat  with  different  specimens  of  urine,  and  is  also  aifeetod  by 
tlie  rate  of  centrifugalisation  emplo3-ed.  Table  44  gives  the  averagt) 
bulk  of  precipitate  ol4a,ined  with  varying  percentages  of  dissolvfo) 
luicinoglobin  in  slightly  diluted  urine.  By  graduating  the  centrifugai] 
tube  cmploj'ed  in  percentages  of  wet  red  cells,  instead  of  in  cubif| 
centimetres,  the  former  could  be  read  ofl  directly  and  all  calculatioii| 
avoided.  It  was  found  that  when  an  equivalent  amount  of  oxalalejj 
blood  jalasma  was  added  (the  red  cells  require  to  be  ?e]raratccl  fron| 
the  blood  plasma  for  convenience  in  hiking)  so  that  (di.s.solvcitj 
haemoglobin  and  blood  ]jlasma  were  present  in  their  natural  rehitkip, 
the  bulk  of  precipitate  obtained  was  increased  by  20  jier  cent,  to  ;ii 
lier  cent. 
'I'aiii.e  44-.  Hulk  iif  precipitate  obtained  on  boiling  to  c.cm.  of  sligbtly  acid  urine  cunlaininj 
varying  percentages  of  dissolved  haemoglobin. 
Hercenfage  of  haeniuglobin  contained  in  urine 
of  sp.  gr.  I '01 4  diluted  with  5  to  25 
of  water. 
Bulk  (after  ccntrlfugalisntlon)  of  precipitate 
obtained  upon  boiling  after  previous 
slight  acidilic.'ition  with  acetic  acid. 
4-0  ",i 
2-0  "o 
'•o”o 
°’5  '/'(i 
0-^5  '!<> 
3 -DO  c.cm. 
2-28  c.cm. 
I  -82  c.cm. 
I '36  c.cm. 
o’i)i  c.cm. 
O' 46  c.cm. 
0-23  c.cm. 
O'  1 1  c.cm. 
I 
-By  one  or  the  other  of  these  methods  the  percentage  a.ud  lot 
amounts  of  haemoglobin  given  in  Tables  43  and  45  were  deteriniiiflil 
In  ten  attacks  of  blackvvater  fever  the  total  amount  of  haei»‘ 
gdobin  passed  into  the  urine  w'as  estimated  (Table  45)  and  was  foi4 
to  be  represented  by  betw^ecn  about  r5  gm.  (Case  7)  and 
(Case  14)  of  w'Ct  red  cells.  In  the  cases  given  in  Table  45  it  will  1*1 
observed  that  there  is  a  general  correspondence  between  t!if| 
]iercentage  and  the  total  amount  of  haemoglobin,  the  higher  tkj 
lormer  the  higher  the  latter.  'hhis  relation,  in  the  light  of  llh 
cx'periments  made  upon  rabbits,  given  in  Table  35,  would  appear  I 
be  merely  a  coincidence.  It  w'dl  be  noted  that  in  the  tw'o  cases 
w'hich  su|.rpression  of  urine  occurred  (7a  and  11)  the  total  amountf^ 
haemoglobin  appearing  in  the  urine,  equivalent  to  g  g.  of  wet  is} 
