187 
At  mid-day  he  had  a  slight  rigor,  and  about  2  p.m.  he  passed 
Burgundy-red  urine.  At  3  p.m.  T.  104-2°  F.  Spleen  enlarged  and 
easily  palpable,  extending  about  two  fingers’  breadth  below  the  costal 
margin. 
I  sth  day.  Patient  was  better  to-day,  and  the  urine  was  amber 
coloured. 
i6th  day.  Patient  quite  well  to-day.  Ilis  recovery  continued 
uninterruptedly. 
Condition  of  blood. — 5th  day.  At  10  a.m.  patient’s  oxalated 
plasma  was  of  a  dark  orange  colour  with  no  red  tint,  containing  o'o6 
per  cent,  of  oxyhaemoglobin.  No  methaemoglobin  bands  were 
observed.  No.isolysin  and  no  iso-agglutination  of  the  red  cells 
was  present.  A  haemocytoineter  enumeration  gave  the  following 
figures: — Red  cells  =  4, 1 40,000  per  mm.^  No  malarial  parasites 
and  no  pigmented  leucocytes  were  found  in  the  blood. 
6th  day.  On  examination  of  blood  films,  no  malarial  parasites  or 
pigmented  leucocytes  were  seen. 
i6th  day.  At  10  a.m.  patient’s  oxalated  plasma  was  of  a  light 
yellow  colour  with  no  red  tint,  containing  O’ 13  per  cent,  of  haemo¬ 
globin.  No  autolysin  present. 
The  following  observations  were  made  at  37°  C.  with  the  patient’s 
red  blood  cells  suspended  in  O'g  per  cent,  sodium  chloride  solution  to 
which  quinine  bihydrochloride  had  been  added  ;  — 
No.  of 
experi¬ 
ment. 
Composition  of  Mi.xture  of  Red  Blood  Cells  and 
Quinine  Solution. 
Haemolysis. 
2'5  %  emulsion  of 
red  blood  cells 
0'9  solution  of 
sodium  diloridc 
1*92  solution  of 
quinine  bihydro¬ 
chloride 
I 
0*65  c.c. 
3’0  c.c. 
O' I  c.c. 
Partial  at  end  of  3  hours. 
2 
0’8  c.c. 
3'6  c.c. 
OM  c.c. 
.Vlmost  complete  at  end  of 
3  hours. 
3 
1*05  c.c. 
^  4-6  c.c. 
O-I  C.C. 
Trace  at  end  of  3  hours. 
Condition  of  urine.—  On  the  ist  day,  in  the  evening  the  urine  was 
of  a  dark  red  colour. 
2nd  day.  The  urine  was  porter  coloured  all  day. 
