2Ir) 
also  distinct.  No  autolysin  was  present  in  the  plasma  and  no  malarial 
parasites  or  pigmented  leucocytes  were  found  in  the  tilood.  On 
examination  with  the  aid  of  the  haemocrit  tlic  blood  was  found  to 
centain  i6'3  per  cent,  by  volume  of  red  cells.  'I'lic  above 
observations  were  made  at  3;°  C.  with  the  patient’s  red  bhiod  cells 
suspended  in  O'g  per  cent,  sodium  chloride  solution,  to  which  quinine 
bihydrochloride  had  been  added. 
4th  day.  At  10  a.m.  patient’s  oxalated  plasma  was  of  a  deep 
orange  colour,  but  not  so  deep  as  yesterday.  It  gave  faint  oxyhaemo- 
globin  bands,  O' 16  per  cent,  of  haemoglobin  being  jmesent  in  solution. 
No  autolysin  was  present  in  the  plasma,  and  no  malarial  para.sites  or 
pigmented  leucocytes  were  found  in  the  blood. 
7th  day.  At  10-30  a.m.  patient’s  o.xalatcd  plasma  was  of  a  light 
orange  colour.  Oxyhaeinoglobin  bands  were  faint,  the  ]jlasnia 
containing  o‘i  per  cent,  of  haemoglobin  in  solution.  No  autolysin 
was  present  m  the  plasma,  and  no  parasites  or  pigmented  leucoc}'tes 
were  found  in  the  blood.  On  examining  with  the  aid  of  the 
haemocrit  the  blood  was  found  to  contain  ly'S  per  cent.  b\-  volume 
of  red  cells.  The  following  observations  were  made  at  37°  C.,  with 
the  patient’s  red  blood  cells  suspended  in  o'g  per  cent,  sodium  chloride 
solution,  to  which  quinine  bihydrochloride  had  been  added  ; 
No.  of 
experi¬ 
ment. 
Composition  of  Mixture  of  Red  Blood  Cells  and 
Quinine  Solution. 
Haemolysis. 
2*5  %  emulsion  of 
red  blood  cells. 
0*9  %  solution  of 
sodium  chloride. 
1*92  %  solution  of 
quinine  bihydro¬ 
chloride. 
I 
0*4  c.c. 
2‘0  c.c. 
0*1  c.c. 
Complete  at  end  of  i  hour 
z 
0'5  c.c. 
2*4  c.c. 
0*1  c.c. 
Complete  at  end  of  i  hour 
3 
0-65  c.c. 
3*0  c.c. 
0*1  c.c. 
Complete  at  end  of  i  .1  hours 
+ 
0-8  c.c. 
3-6  c.c. 
o-i  c.c. 
Complete  at  end  of  2  hours 
5 
1*05  C.C. 
3-6  c.c. 
O' I  C’C. 
Trace  at  end  of  3  hours 
6 
1*3  c.c. 
5-6  c.c. 
O' I  c.c. 
Nil  at  end  of  3  hours 
7 
1*5  c.c. 
6-4. c.c. 
O' I  c.c. 
Nil  at  end  of  3  hours 
8 
1*3  c.c. 
4-6  +  r  c.c.  plasma 
O'l  c.c. 
Nil  at  end  of  3  hours 
ConJ-ition  of  the  spleen. —  6th  day.  At  ii  a.m.  a  spleen  puncture 
was  made.  Phagocytosis  of  red  cells  (by  less  than  o'l  per  cent,  of 
the  white  cells)  was  observed.  No  malarial  parasites  or  pigment 
found. 
