227 
present  in  the  plasma,  and  no  malarial  parasites  or  pigmented 
leucocytes  were  found  in  the  blood.  With  the  aid  of  the  haemocrit 
the  patient’s  blood  was  found  to  contain  33-3  per  cent,  by  volume, 
Fig.  78.  Blackwater  Fever,  Case  14.  Temperature  Chart. 
of  red  cells.  The  following  observations  were  made  at  37°  C.  with 
the  patient’s  red  blood  cells  suspended  in  o’p  per  cent,  sodium 
chloride  solution,  to  which  quinine  bihydrochloride  had  been  added  : 
No.  of 
experi¬ 
ment. 
Composition  of  Mixture  of  Red  Blood  Cells  and 
Quinine  Solution. 
Haemolysis. 
2-5  %  emulsion  of 
red  blood  cells 
0*9  %  solution  of 
sodium  chloride 
1-92  %  solution  of 
quinine  bihvdro- 
chloride 
I 
0*5  c.c. 
2*4  c.c. 
0*1  c.c. 
Complete  at  end  of  i  hour 
2 
0*65  c.c 
3-0  c.c. 
0*1  c.c. 
Complete  at  end  of  2  hours 
3 
0*8  c.c. 
3-6  c.c. 
OM  c.c. 
Complete  at  end  of  2  hours 
+ 
i‘05  c.c. 
4*6  c.c. 
0*1  c.c. 
Trace  at  end  of  3  hours 
5 
1-3  c.c. 
5*6  c.c. 
0*1  c.c. 
Nil  at  end  of  3  hours 
ConEilion  of  spleen. — 4th  day.  At  5-30  p.m.  a  spleen  puncture 
was  made.  Phagocytosis  of  red  cells  (by  less  than  i  per  cent,  of 
white  cells)  was  observed.  No  malarial  parasites  or  pigment  found. 
