232 
This  was  the  first  intimation  he  had  that  he  was  suffering  from 
blackwater  fever.  He  had  no  previous  rigor.  T.  I03‘6°  F. 
2nd  day.  Patient  had  a  bad  night,  vomiting  very  troublesome, 
skin  of  a  marked  yellowish  brown  colour.  T.  103°  F.  Twenty-three 
ounces  of  physiological  saline  solution  were  injected  subcutaneously. 
3rd  day.  General  condition  improved  ;  the  vomiting  was  not  so 
troublesome,  and  the  temperature  had  fallen  to  100°  F.  Twenty- 
three  ounces  of  physiological  saline  solution  were  again  injected 
subcutaneously. 
4th  day.  Patient  was  much  better,  and  his  recovery  continued 
uninterruptedly. 
Condition  of  blood.-  -ist  day.  At  9-30  p.m.  patient’s  oxalated 
plasma  was  of  a  dark  orange  colour  with  a  faint  red  tint,  containing 
o'56  per  cent,  of  haemoglobin.  No  autolysin  was  present  in  the 
plasma,  and  no  parasites  or  pigmented  leucocytes  were  found  in  the 
blood.  The  blood  had  a  watery  appearance.  The  following 
observations  were  made  at  37°  C.  with  the  patient’s  red  blood  cells 
suspended  in  eg  per  cent,  sodium  chloride  solution  to  which  quinine 
bihydrochloride  had  been  added:  — 
No,  of 
experi¬ 
ment. 
Composition  or  Mixture  of  Red  Blood  Cells  and 
Quinine  Solution. 
Haemolysis. 
2*5  %  emulsion  of 
red  blood  cells 
0*9  %  solution  of 
sodium  chloride 
1*92  %  solution  of 
quinine  bihydro¬ 
chloride 
I 
0*5  c.c. 
2*4  c.c. 
0‘i  c.c. 
Complete  at  end  of  i  hour 
2 
0-65  c.c. 
3*0  c.c. 
o-i  c.c. 
Complete  at  end  of  2  hours 
3 
0-8  c.c. 
3-6  C.C. 
0‘i  c.c. 
Complete  at  end  of  zjhoufj 
4 
1*05  c.c. 
4*6  c.c. 
0*1  c.c. 
Trace  at  end  of  3  hours 
5 
1-3  C.C. 
5-6  C.C. 
O'T  c.c. 
Nil  at  end  of  3  hours 
2nd  day.  At  i  p.m.  patient’s  oxalated  plasma  was  of  a  dark 
orange  colour  with  no  red  tint,  containing  0'42  per  cent,  of  haemo¬ 
globin.  No  autolysin  was  present  in  the  plasma. 
Condition  of  the  spleen. —  ist  day.  At  10  p.m.  a  spleen  puncture 
was  made.  Phagocytosis  of  red  cells  (by  less  than  i  per  cent  o( 
white  cells)  was  observed.  No  malarial  parasites  or  pigment  found 
