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case,  two  to  a  field)  from  three  bovines,  without  result.  The  owner 
had  very  kindly  written,  giving  us  permission  to  take  away  an 
infected  sheep  or  goat  that  the  strain  might  be  carried  to  our  camp 
at  Kambole ;  and  a  sheep  which  showed  trypanosomes  at  both  visits 
was  selected.  We  take  this  opportunity  of  thanking  Mr.  Yule  for 
his  consideration,  without  which  even  the  little  possible  could  not 
have  been  done. 
This  sheep  was  carried  in  an  improvised  hammock  for  ten  days, 
when  it  was  found  to  have  lost  its  condition  and  become  very  weak. 
On  the  twelfth  it  was  in  extremis  and  was  destroyed  (September 
1 2th),  and  S'oc.cm.  of  blood  containing-  trypanosomes  one  to  a  field 
was  injected  into  a  Sheep  No.  i,  which  had  been  purchased  in  a 
healthy  village  and  been  examined  by  blood  and  gland  puncture  on 
three  occasions  with  negative  results.  This  sheep,  which  became 
infected  about  the  eighth  day,  reached  Kambole  on  September  2ist, 
and  served  as  the  source  of  the  strain. 
The  following  is  the  genealogy  of  this  strain :  — 
SCOTSD.VLE  SHEEP 
Mouse 
Rat 
2a 
Grey  rat 
Rat 
Sa 
Guinea-pig  Guinea-pig 
3  7 
Experimental 
Sheep  I 
Guinea-pig  Sheep  \ 
8  (arrived  at 
Pretoria) 
Rat  1 1  Guinea-pig 
15 
Dog  5 
(arrived  at 
Pretoria) 
Rabbit 
(inoculated  at 
Pretoria) 
Goat 
The  inoculations  here  described  are  those  carried  out  with  the 
original  Scotsdale  sheep,  or  its  passage  through  Sheep  i. 
R.\ts. — Two  white  rats  received,  the  one.  No.  2a,  6-o  c.cm.  (24.9.08),  the  other, 
5^1)  S'o  c.cm.  (22.10.08)  intraperitoneally,  of  blood  containing  numerous 
trypanosomes  from  Sheep  i.  One  white  rat.  No.  ii,  received  lo-o  c.cm.  (25.10.08) 
intraperitoneally  from  Sheep  4  (inoculated  from  Sheep  i)  trypanosomes  one  to 
a  field.  None  of  these  rats  became  infected. 
f 
