342 
Guinea-pigs.— Three  guinea-pigs  were  inoculated  intraperitoneally  from 
Sheep  i;  No.  3,  6-o  c.cm;  No.  7,  30-0  c.cm;  No.  8,  20-0  c.cm;  and  one,  iNo.  15, 
with  15-0  c.cm.  from  Sheep  4-  None  of  these  became  infected. 
A  Wild  Mouse  (Sp.  ?)  received  0-4  c.cm.  subcutaneously,  and  a  Grey  Rat 
3-0  c.cm.  intraperitoneally  from  Sheep  i.  The  mouse  escaped  on  the  i8th  day, 
ini  had  not  up  till  then  shown  trypanosomes.  Ihe  rat  was  under  observation  for 
two  months  and  trypanosomes  were  never  seen. 
Radbit.— We  were  unable  to  utilise  rabbits  in  Rhodesia,  but  on  arrival 
in  Pretoria  a  rabbit  was  inoculated  intraperitoneally  with  S’O  c.cin.  citrated  blood 
o  Sheep  4.  Trypanosomes  appeared  on  the  seventh  day  J  urther  observations 
Tre  necessary  to  determine  the  constancy  of  this  reaction  m  rabbits  but  there  is 
no  reason  to  consider  that  any  contamination  of  this  strain  had  been  effected 
during  the  journey. 
ryoG  4  —An  adult  ‘  Kaffir  ’  dog  was  inoculated  intraperitoneally  on  October 
,.„d  .J:  with  .0-0  c.cm,  cttated  blood  of  Sheep  The  temperalme  beca», 
irteeular  ftom  the  eighth  day  and  reached  103-6  on  the  evening  of  the  thirteenth, 
during  the  daily  eitaminations  to  November  .5th  no  trypanosomes  were  ever  seen 
“then  in  the  peripheral  blood  or  on  gland  puncture,  and  the  dog  remained  health, 
iraDoearance  On  November  a-th  one  trypanosome  was  seen  in  a  «.inch  covo- 
gjaS  preparation.  From  that  date  to  January  noth,  at  Pretoria,  they  were  not 
again  seen.  _  ,  ,, 
Sheep— A  naturally  infected  sheep  was  presented  to  the  Expedition  by  ilr, 
T  B  ?ule  of  Scotsdale  and  constituted  the  origin  of  this  strain.  It  was  in 
air  conditimr  at  the 'time  of  leaving  Scotsdale.  but  rapidly 
travelling,  though  carried  in  a  hammock,  and  was  destroyed,  when  exlremts, 
on  September  12th. 
Sheep  i. -September  12th,  1908,  inoculated  subcutaneously  with  S'o  cxm^ 
blood  direct  from  the  original  animal.  Organisms  appeared  about  the  ei^bth 
niv  It  was  carefully  carried  to  our  camp  at  Kambole  and  on  arrival  there 
September  send,  showed  trypanosomes.  The  temperature  was  elevated  an 
irrLular  and  did  not  show  the  striking  picture  manifested  by  a  chart  the 
Broken  Hill  T.  dimorihon.  Organisms  were  present  daily  'fom 
and  were  then  absent  for  ten  consecutive  days,  reappearing  on  October  11  h.  Fr 
October  1st  the  temperature  assumed  a  much  more  irregular  type  which 
persisted  to  October  23rd  when  the  animal  died  of  septic  pleuro-pneiimoma. 
Sheep  4 -October  loth,  1908.  Inoculated  intraperitoneally  with  30-0  c.cin 
citrated  blood  of  Sheep  i.  This  large  dose  was  given  as  trypanosomes  had  no 
been  seen  for  ten  days. 
Organisms  appeared  on  the  sixth  day  and  have  been  almost  constantly 
present  since,  though  always  in  small  numbers,  it  being  rare  to  see  n,any  a 
one  to  the  field,  more  commonly  one  to  five  or  ten  fields  being  n 
The  temperature  showed  little  abnormality,  and  no  paroxysmal  tendency. 
This  sheep  (‘long-eared’)  was  carried  to  Pretoria  where  it  is  under  the  care 
of  Dr.  Theiler  who,  under  date  of  April  26th,  writes  that  it  is  still  alive. 
Goat  a.-Inoculated  subcutaneously  with  5-0  c.cm.  ^h^P 
22nd  The  temperature  suddenly  rose  to  105-7°  the  evening  of  the  tenth  day,  an 
:rp'«„rir„g  =rt:r„b::vver;Tr '^mV^ 
susceptible  on  the  twenty-second  day. 
