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and  their  ‘  animal  reactions,’  that  they  might  be  of  service  in  the 
transmission  experiments  we  contemplated. 
The  history  of  the  cattle  on  this  station  is  of  interest.  Between 
1896,  when  the  Mission  was  established,  and  1905,  cattle,  sheep,  goats 
and  pigs  aie  reported  to  have  done  exceedingly  well.  In  1905  the 
herd  of  nearly  60  head  was  divided,  half  going  to  the  Luapula  River 
where  they  have  since  done  well,  the  others  remaining  at  Kambole! 
The  owner  of  the  latter  half  was  away  in  May  and  June  of  this  year 
and  SIX  weeks  after  his  return  (i.e.  August)  the  cattle  commenced  to’ 
die,  and  twenty  were  lost  before  Christmas.  In  1906  four  deaths  are 
remembered,  in  1907  six,  and  up  to  our  visit  in  1908  seven  had  died 
Several  additions  to  the  herd  had  been  made  by  movements  from 
other  stations  of  the  same  Missionary  Society,  e.g.,  Niamkolo  and 
Kawimbi,  bringing  the  number  of  adults  present  at  the  time  of 
examination  up  to  15.  Of  these  six  were  infected.  The  herdman 
who  brought  the  original  stock  from,  the  North  end  of  Lake  Nyasa 
m  1896  IS  still  at  Kambole,  and  he  is  unable  to  offer  any  explanation 
as  to  vvhy  cattle  did  so  well  and  Nvere  free  from  disease  up  to  1905, 
and  have  since  died  in  such  numbers.  He  avers,  and  the  local  natives 
support  Inm,  that  these  cattle  have  always  grazed  on  the  same  areas, 
and  that  ‘  tushembe  ’  was  always  to  be  found  if  the  animals  wandered 
far.  Unfortunately  the  name  ‘  tushembe  ’  is  applied  here  to  all  biting 
flies,  and  the  natives  do  not  recognise  or  distinguish  Glossina,  for 
which  this  vernacular  word  is  usually  restricted.  It  is  possible  that 
an  extension  of  tsetse  may  have  occurred  without  notice.  At  the 
present  time  Gl.  morsitans  may  be  taken  within  three  or  four  miles 
to  the  West,  and  wandering  flies  were  captured  on  the  Mission  during 
our  stay  near  there. 
Two  of  the  infected  cattle — ‘  Balungu  ’  and ‘Nmamwenda  ’ — -were 
selected  for  the  isolation  of  the  strains,  since  the  morphological 
appearances  of  their  trypanosomes  sho\ved  some  points  of  difference. 
A.  ‘  Balitngu  ’  strain. 
This  six-year-old  cow  wms  born  at  Kambole.  She  showed  the 
hide-bound  dejected  appearance  of  a  chronic  case,  with  slightly 
anaemic  membranes  and  enlarged  superficial  lymphatic  glands,  but  no 
oedemata.  She  was  examined  on  five  occasions,  trypanosomes  being 
present  m  the  peripheral  blood  on  each,  and  she  was  destroyed 
