werr. 
m 
350 
in  extre^nis  on  November  7th,  igo8,  having  been  sick  for  approxi¬ 
mately  nine  months. 
Autofsy.  All  subcutaneous  tissues  were  pale  and  somewhat  dropsical.  The 
superficial  glands  were  considerably  enlarged  and  oedematous.  The  abdominal 
cavity  contained  a  slight  excess  of  deep  citron-coloured  fluid.  The  peritoneal 
covering  of  the  rumen,  reticulum,  spleen  and  areas  of  the  intestine  was  studded 
with  petechiae,  in  parts  congregated  into  patches  two  inches  in  diameter.  The 
mesentery  was  infiltrated  with  a  gelatinous  material.  ihe  liver  was  slightly 
conge.sted;  the  gall-bladder  distended  and  contained  over  900  c. cm.  of  reddish  walerr- 
bile.  The  spleen  was  somewhat  enlarged,  the  surface  studded  with  petechiae,  the 
pulp  dark  and  friable,  and  Malpighian  bodies  prominent. 
There  was  no  excess  of  fluid  in  the  thoracic  cavity  or  pericardial  sac.  Slight 
lobar  congestion  and  emphysema  of  both  lungs.  The  heart,  pale  and  very  flabby, 
.showed  fatty  degeneration;  the  base  was  surrounded  by  a  quantity  of  gelatinous 
material  replacing  all  fat. 
The  mesenteric  and  mediastinal  lymphatic  glands  were  enlarged  and 
oedematous  and  some  of  them  were  congested. 
The  following  is  the  genealogical  record  of  this  strain  :  — 
BALUNGU 
Rat  Guinea-pig 
7  5 
Guinea-pig 
9 
Dog 
-> 
0 
1 
Sheep  5  Dog  Goat 
(arrived  at  8  5 
Pretoria) 
Guinea-pig 
>  + 
Dog 
/5 
(arrived  at 
Pretoria) 
Experimenlal  infect  ion 
Hat  7. — Considering  it  possible  we  might  be  dealing  with  ‘  .Scotsdale’ 
Strain,  10  c.cm.  blood  of  naturally  infected  cow  was  inoculated  intraperitoneally 
into  this  animal,  on  October  23rd.  Negative  on  the  29th;  trypanosomes  were 
seen  on  November  ist  and  remained  present  until  death  on  November  loth— 17 
days. 
rrUiNEA-PiG  i;. — October  8th,  1908.  Inoculated  intraperitoneally  with  5  c.cm. 
blood  of  naturally  infected  cow.  Almost  daily  examination  up  to  November  10th 
failed  to  reveal  any  infection,  so  the  animal  was  re-inoculated  on  this  date  with 
about  2-0  c.cm.  heart  blood  of  Rat  7  just  dead.  Trypanosomes  were  seen  on 
November  23rd,  the  first  examination  since  November  13th,  and  were  present  at 
succeeding  observations  till  death  on  December  12th. 
Guinea-pig  9. — Owing  to  the  initial  failure  to  obtain  infection  in  Guinea-pig 
5,  this  animal  received  lo-o  c.cm.  intraperitoneally  on  October  23rd.  Trypanosomes 
first  seen  November  ist;  they  were  not  seen  on  the  5th  or  7th,  but  thereafter 
were  constantly  present — but  always  in  small  numbers,  2  in  of  a  cover  inch) 
being  the  maximum  noted  until  a  week  prior  to  death,  which  took  place  on 
December  4th  {41  days),  when  t/hey  became  more  numerous,  up  to  two  per  field, 
