351 
Guinea-pig  i4.-December  12th.  Inoculated  intraperitoneally  with  o-c  ecu 
s  n;:  sr' "  “  f  r 
accelerated  by,  if  not  due  to  a  vegetable  poisouiug  whicra’cco"  Medio  ^h^fola 
of  three  other  guinea-pigs  the  following  day. 
DOG  3.-October  8th,  1908.  Inoculated  intraperitoneally  with  s'o  c.cm  blood 
of  Halungu.  I  he  temperature  rose  to  102°  on  the  fourfh  day.  and  cltinued 
slightly  elevated  between  102°  and  103°.  with  few  excepLus.  to  death 
Trypanosomes  appeared  i  in  14  cover  on  the  eighth  day.  and  remained  preseni 
in  small  numbers,  ,  in  10  fields  being  the  maximum,  up  to  November  i,th  then 
r,  "“IIS. " — «.b  da!; 
Dod  8.— October  gtst.  Inoculated  subcutaneously  with  ro  c.cio.  blood  of 
Halungu.  demperature  rose  almost  immediately  to  .os”  and  continued  irre-ula 
so  long  as  tt  was  taken.  Trypanosomes  were  never  seen  in  the  blood,  but  glald 
puncture  on  the  toth  and  ,pth  day  after  inoculation  showed  them  to  be  prescM 
Death  took  place  on  December  4th  (35  days)  from  pneumonia.  Trypano.oLs 
could  not  be  demonstrated,  and  the  spleen  was  normal  '  ^ 
dog  is.-November  asth.  Inoculated  subcutaneously  with  3.0  c.cm  heart 
blctod  of  Dog  3  JU.S  dead.  Trypanosomes  were  never  seen  in  the  blood  or  gland 
juice  of  this  animal  up  to  his  arrival  in  Pretoria.  ^ 
hlnofrrp  intraperitoneally  with  6-o  c  cm 
blood  of  Balungu.  Trypanosomes  were  seen  i  in  i  cover  on  the  sixth  day  and 
were  subsequently  found  in  small  numbers  (i  in  i  on  one  occasion  only)  The 
temperature  showing  indications  of  the  Broken  Hill  type,  but  les! 
taken  to  Pretoria,  and  was  still  livinfon  January 
23rcL  Dr.  Theiler  informs  us  on  April  26th,  1909,  that  this  sheep  h!s  since  died 
fbl  ^'^0^'^tated  subcutaneously  with  ro  c.cm.  blood 
of  Balungu.  The  temperature  rose  steadily  and  reached  106-4°  on  the  evening  of 
the  eighth  day  and  continued  irregular.  Trypanosomes  were  not  seen  in  ^he 
peripheral  blood  until  the  14th  day  (i  in  cover);  they  had,  however,  been  seen 
was  LS.'  destroyed  when  the  infection 
Morphology  of  '  Balungu  ’  irypano  some. 
The  trypanosomes  seen  in  the  original  cow  and  the  sub-inoculaled 
animals,  rat,  guinea-pigs,  dogs,  sheep  and  goat,  are  of  the  same 
appearance.  They  correspond  closely  with  the  tadpole  form  of 
T.dimorphon  (Plate  IV,  fig.  3). 
In  fresh  preparations  the  movements  are  localised  to  the  field,  and 
are  not  very  active. 
In  stained  preparations  the  length  varies  from  io-2  to  16-3  fx 
(average  13  ^),  the  larger  being  divisional  form,  and  the  breadth  from 
I'O  to  2-2  ,x  (average  1-5).  The  following  15  the  mean  of  a  series  of 
measurements :  — 
Extremity  to 
Nucleus 
Nucleus  to  flagellar 
Nucleus  extremity 
2-07  6-o8 
4-93 
