355 
were  present  at  each  succeeding  examination  till  our  departure.  The 
owner  and  the  herdsman  considered  her  to  have  recovered  her 
original  good  health,  and  said  she  gave  a  normal  amount  of  milk  and 
nourished  her  calf  well.  The  following  inoculations  were  made  from 
this  animal :  — 
NINAMWENDA 
Mouse  Guinea-pig  Dog 
Sheep 
1 
Sheep  6 
1 
Goat  3 
6  2 
3 
(arrived  in 
(arrived  ir 
Experimental  infection 
Pretoria) 
Pretoria) 
Ox 
2 
Grey  mouse  caught  locally.  Inoculated  October  23rd  with  o'4  c.cm.  direct 
from  Ninamwenda.  No  trypanosomes  were  seen  in  its  blood  up  to  November 
6th,  when  it  escaped. 
No  EATS  were  available  for  inoculation. 
Guinea-pig.  October  8th,  igo8.  Inoculated  intraperitoneally  with  lo-o  c.cm. 
blood  of  Ninamwenda.  Trypanosomes  were  not  seen  at  almost  daily  examinations 
until  November  3rd— the  26th  day.  They  disappeared  for  five  days  then 
returned,  being  present  at  each  subsequent  examination  in  numbers  varying  from 
I  in  %  cover-glass  to  i  in  a  field — an  average  would  be  one  to  eight  or  ten  fields. 
The  animal  maintained  its  condition  well,  despite  the  rough  usage  and  exposure 
on  the  march,  and  was  accidentally  killed  on  January  3rd,  1909.  There  were  no 
abnormal  lesions  on  autopsy,  and  the  body  was  well  nourished  and  fat. 
Dog  2. — October  8th,  1908.  Inoculated  intraperitoneally  with  lo-o  c.cm.  blood 
of  Ninamwenda.  From  the  8th  day  the  temperature  became  irregular  and  jerky, 
and  the  dog  lost  condition,  acquired  a  harsh  coat  and  appeared  depressed.  A 
month  after  inoculation  there  was  considerable  oedema  of  the  sub-maxillary  space 
and  a  catarrhal  discharge  from  the  eyes.  He  died  in  an  emaciated  state  on 
November  29th  (52  days),  without  ever  showing  trypanosomes  in  his  blood  or  on 
gland  puncture.  Post-mortem  showed  only  general  enlargement  of  all  lymphatic 
glands,  and  considerable  oedema  in  the  connective  tissues  of  throat  and  in  the 
body  cavities.  The  spleen  appeared  normal.  No  sub-inoculations  were  possible, 
but  a  careful  search  failed  to  show  trypanosomes  in  any  organ. 
Sheep  3. — An  aged  male.  October  8th,  1908.  Inoculated  subcutaneously  with 
I'o  c.cm.  blood  of  Ninamwenda.  Trypanosomes  first  appeared  on  the  23rd  day 
at  which  time  the  temperature  rose  slightly.  They  were  then  present  daily,  with 
two  exceptions,  till  the  animal  was  destroyed  on  our  leaving  for  home.  They 
were  never  numerous — i  to  two  fields  being  highest  recorded,  and  the  temperature 
was  but  slightly  irregular,  io4‘8°  maximum. 
Sheep  6. — October  23rd,  1908.  Inoculated  intraperitoneally  with  3-0  c.cm. 
blood  of  Ninamwenda.  Trypanosomes,  1  io  %  cover-glass,  were  seen  in  the  iith  day; 
on  the  12th,  13th,  14th  they  were  absent,  but  reappeared  on  the  15th,  from  which 
day  they  were  always  present  in  small  numbers — i  to  10  fields  maximum.  The 
temperature  showed  very  little  irregularity,  and  the  animal  travelled  well, 
reaching  Pretoria  in  January  in  .  good  condition,  and  according  to  Dr.  Theiler’s 
letter  of  April  26th,  was  then  still  alive. 
Goat  3. — About  three  months,  still  with  its  mother.  October  23rd,  1908. 
Inoculated  intraperitoneally  with  3-0  c.cm.  blood  of  Ninamwenda.  One 
trypanosome  was  seen  on  the  19th  day,  but  not  again  during  the  regular 
examinations.  On  the  march  they  were  seen  twice  in  five  observations  and  were 
