35^ 
nresent  on  this  animal’s  arrival  in  Pretoria.  There  was  no  apparent  loss  in 
condition  and  no  symptoms  were  manifested,  and  this  animal  was  alive  on  April 
0x^2^— ri  years  old.  October  23rd,  1908.  Inoculated  subcutaneously  with 
.-o  c.cm.  blood  of  Ninamwenda.  The  temperature  rose  on  the  eighth  day  to 
102°  and  continued  in  that  neighbourhood  to  the  17th,  when  it  rose  to  105-2“, 
failine  again  on  the  25th  when  temperatures  were  ceased.  Trypanosomes  were 
seen  in  the  blood  on  the  loth  day,  and  were  thereafter  present  daily,  ranging 
from  I  to  3  to  a  field.  Gland  puncture  had  shown  the  infection  three  day.s 
prior  to  their  appearance  in  the  blood.  No  symptoms  were  noticed  in  the 
animal  during  the  three  and  a  half  weeks  of  observation,  and  we  have  received 
no  further  report  as  to  his  present  condition.  1  ,  iv 
At  Pretoria,  inoculations  were  made  from  both  Sheep  6  and  Goa  3.  Dr. 
Theiler  informs  us  on  April  26th  that  from  the  former  animal  both  the  g^mea-pig 
and  the  rabbit  became  infected,  from  the  latter  the  guinea-pig  is  positive,  but  the 
rabbit  is  negative. 
Morphology  of  the  '’Ninamwenda'  Trypanosome 
In  fresh  preparations  the  trypanosomes  from  this  cow  and  also 
from  a  second  (Nakakoti)  were  clearly  distinct  from  those  of  the 
T.  congolense  type  seen  in  Balungu.  They  appeared  as  slightly 
elongated,  egg-shaped  bodies,  moving  across  the  field  with  the 
flagellar  extremity  in  front,  and  rarely  showing  any  movement  of  the 
body,  even  when  held  up  by  corpuscles.  They  easily  traversed  the 
field  of  vision,  but  at  a  rate  at  which  they  were  readily  followed. 
Stained  by  Leishman’s  method,  they  measured  from  ii  to  19/1 
in  length,  the  latter  representing  divisional  forms  (average  14-66  /i), 
and  from  2-9  to  3-75  in  breadth  (average  3-38  y).  The  mean  of  a 
series  of  measurements  is; 
Extremity  to  Nucleus  to  Portion  of  free 
Nucleus  Nucleus  flagellar  extremity  flagellum 
5.45  2-36  5-1  '-7 
(In  Nakakoti)  4-97  ^ 
In  Nakakoti  the  average  width  was  3-58. 
The  blepharoplast  is  almost  terminal  or  up  to  0-8  y  from  the  end. 
The  rounded  or  oval  nucleus  is  almost  centrally  placed.  The  undulating 
membrane  is  represented  by  a  narrow  band  running  parallel  to  the 
body,  being  better  developed  in  the  narrower  forms.  The  rather 
well-defined  rim  is  continued  as  a  bristle-like  projection  of  up  to 
r;  in  length,  hardly  amounting  to  a  flagellum,  but  free  of  ectoplasm. 
The  posterior  extremity  is  rounded  or  very  bluntly  angular,  the 
flagellar  abruptly  drawn  out.  The  cytoplasm  stains  a  deep  pink,  and 
commonly  shows  a  large  and  most  distinct  vacuole  just  posterior  to 
the  nucleus.  Though  not  always  present,  or  not  so  prominent,  this 
